Injectable chitosan composite hydrogel capable of promoting myocardium repair and preparation method of injectable chitosan composite hydrogel
A technology of composite hydrogel and chitosan, which is applied in medical science, prosthesis, etc., can solve the problems of poor biocompatibility and poor mechanical properties of injectable hydrogel, and achieve good biocompatibility, softness and hardness. Controllable and repeatable results
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Embodiment 1
[0043] (1) Extraction of human hair keratin
[0044] a) Weigh 2g of human hair test sample (purchased from a barber shop), cut into fragments of about 2cm, and place them in a 400mL Erlenmeyer flask;
[0045] b) Add 100 mL of peracetic acid with a mass concentration of 2%, and shake at 150 rpm on a shaker at 37°C for 24 hours;
[0046] c) Filter through a sieve with a pore size of 350 μm, rinse the hair test sample with ultrapure water, and remove excess oxidant;
[0047] d) Treat with 200mL of 100mM Tris solution on a shaker at 37°C at 150rpm for 2h, filter through a 350μm sieve, and retain the filtrate, continue processing with 200mL of ultrapure water for 1.5h, filter through a 350μm sieve, and retain the filtrate;
[0048] e) The two filtrates are combined, centrifuged at 6000 rpm for 10 min to remove insoluble matter, and then filtered with filter paper;
[0049] f) Dialyze with a dialysis bag (molecular weight cut-off 3K) for 24 hours, concentrate the solution, and fre...
Embodiment 2
[0060] The difference between this example and Example 1 is: take 0.1mL sodium β-glycerophosphate solution, add 10 μL keratin solution, mix well, add into 1mL chitosan solution, stir evenly until the solution is clear without turbidity. Add 0.3 mL of genipin solution, stir evenly, and let stand to obtain chitosan / keratin composite hydrogel. Others are the same as in Example 1. The gel time of this embodiment is 2min.
Embodiment 3
[0062] The difference between this example and Example 1 is: take 0.1mL sodium β-glycerophosphate solution, add 20 μL keratin solution, mix well, add 1mL chitosan solution, stir evenly until the solution is clear without turbidity. Add 0.1 mL of genipin solution, stir evenly, and let stand to obtain chitosan / keratin composite hydrogel. Others are the same as in Example 1. The gel time of this embodiment is 6min.
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