Method for simultaneously determining content of three effective components in turmeric products

A technology of active ingredients and content, which is applied in the field of simultaneous determination of three active ingredients in turmeric products, can solve the problems that it is difficult to fully reflect the quality and efficacy of curcumin, and the single index of curcumin, so as to provide work efficiency, simple method, and reduce The effect of times

Inactive Publication Date: 2015-09-16
SICHUAN NEO GREEN PHARMA TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, curcumin is used as a single indicator for the determination of the content of turmeric medicinal materials and its products, which is difficult to fully reflect its overall quality and efficacy

Method used

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  • Method for simultaneously determining content of three effective components in turmeric products
  • Method for simultaneously determining content of three effective components in turmeric products
  • Method for simultaneously determining content of three effective components in turmeric products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Carry out the preparation of reference substance solution and need testing solution according to the steps of the present invention, and measure.

[0040](1) Preparation of reference substance stock solution: Accurately weigh curcumin, demethoxycurcumin, bisdemethoxycurcumin, mix them, add methanol solution to prepare concentrations of 98.88, 44.40, 25.92 μg respectively / mL of the mixed reference substance stock solution.

[0041] (2) Preparation of reference substance solution: Accurately measure 0.3mL, 0.6mL, 1.2mL, and 2.5mL of the above-mentioned stock solutions, and dilute them to 5mL with methanol solution respectively to obtain mixed solutions of reference substances with different concentrations.

[0042] (3) Preparation of the test solution: take 0.2 g of the sample to be tested, add 25 mL of methanol to dissolve it, weigh it, and ultrasonically process it for 30 minutes at 250 W and a frequency of 40 kHz, let it cool, then weigh it, and use methanol Make up ...

Embodiment 2

[0063] In order to test the reliability of the above experiments and the accuracy of the linear regression equation, the following precision experiments, repeatability experiments, stability experiments and sample recovery experiments were set up, and the stability of the experimental method was affected by the RSD value of each experiment. However, it should be stated that the following experiments do not affect the testing methods and results of the present invention.

[0064] 1. Precision test

[0065] Precisely draw 10 μL of the above mixed reference substance stock solution, repeat the injection 6 times, measure the peak area of ​​curcumin, demethoxycurcumin, bisdemethoxycurcumin, and calculate the RSD value of the repeated 6 injection samples. The precision test results are shown in Table 5:

[0066] Table 5 Precision test results

[0067]

[0068] As can be seen from Table 5, when repeating 6 injections, the RSD values ​​of curcumin, demethoxycurcumin, and bisdemet...

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Abstract

The invention relates to a method for simultaneously determining the content of three effective components in turmeric products and belongs to the technical field of a medicine quality determination method. The method comprises the following steps of: (1) preparing a comparison product stock solution; (2) preparing the comparison product solution; (3) preparing a test sample solution; (4) determining by a high performance liquid chromatography; and (5) calculating the content of a sample to be detected. According to the method, the high performance liquid chromatography is used for simultaneously determining the content of the three effective components including curcumin, demethoxycurcumin and di-demethoxycurcumin in the turmeric products; compared with the single determination on the content of the curcumin in the prior art, the quality and the medicine effect of turmeric medicinal materials and the products can be accurately controlled; and the method has the characteristics of simplicity, rapidness, high efficiency, economical efficiency, comprehensiveness and the like. The method is verified through a precision experiment, a repeatability experiment, a stability experiment and a sample adding recycling rate experiment, and has very good reliability and accuracy.

Description

technical field [0001] The invention belongs to the technical field of drug quality determination methods, in particular to a method for simultaneously determining the contents of three active ingredients in turmeric products. Background technique [0002] Traditional Chinese medicine has the characteristics of multi-component, multi-target, multi-level, and overall effect. However, at present, the content of a single index component in the medicinal material is often used as the evaluation index for its quality control, which is difficult to accurately and comprehensively reflect its quality, let alone comprehensively reflect its functions and effects. Turmeric is the dry rhizome of Curcuma longa L., a plant of the Zingiberaceae family. It is a traditional medicinal material for both medicine and food. , Lump in the abdomen, rheumatic shoulder and arm pain, tumbling and swelling pain, etc. At present, curcumin is used as a single indicator for the determination of the con...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 周厚成胡昌江李文兵吴文辉陈咏梅冯健
Owner SICHUAN NEO GREEN PHARMA TECH DEV
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