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Bacillus amyloliquefaciens strain for prevention and treatment of tomato bacterial wilt and microbial organic fertilizer thereof

A technology for microbial organic fertilizer and starch spore-resolving, applied in microorganisms, organic fertilizers, microorganism-based methods, etc., can solve the problems of crop wilting and death, loss of nutrient resources, waste of organic matter resources, etc., to control tomato bacterial wilt disease, reduce Pesticide residues, significant antagonistic effects

Active Publication Date: 2015-09-16
湖南慧谷农业生态研究院有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Once the disease occurs, it is difficult to control, often causing large-scale wilting and death of crops or even crop failure, resulting in a serious decline in tomato yield, which seriously restricts the development of the tomato industry and the improvement of economic benefits.
At present, there are various methods for the prevention and treatment of tomato bacterial wilt at home and abroad, but because R. solanacearum is a complex group with obvious physiological differentiation, strains from different regions and different host sources differ in host range, pathogenicity, There are great differences in biological characteristics such as biochemical type and serotype, a wide range of distribution, a wide range of hosts, and strong survival and transmission capabilities. There is no better control method
[0003] On the other hand, crop stalks in agricultural production are generally burned on the spot, and livestock and poultry manure after large-scale breeding are discarded anywhere, etc., causing a large amount of nutrient resources to be lost outside the soil-soil system, which not only seriously pollutes the environment, but also greatly affects the environment. The waste of organic matter resources that can be used as raw materials for the production of organic fertilizers and microbial organic fertilizers is not conducive to the sustainable development of my country's agriculture

Method used

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  • Bacillus amyloliquefaciens strain for prevention and treatment of tomato bacterial wilt and microbial organic fertilizer thereof
  • Bacillus amyloliquefaciens strain for prevention and treatment of tomato bacterial wilt and microbial organic fertilizer thereof
  • Bacillus amyloliquefaciens strain for prevention and treatment of tomato bacterial wilt and microbial organic fertilizer thereof

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0034] The isolation and identification of embodiment 1 bacterial strain

[0035] Obtaining strains: Antagonistic bacteria were isolated from the rhizosphere soil of healthy tomato plants in tomato bacterial wilt diseased fields. Then through the pot test, the high-efficiency antagonistic strain T-5 was finally screened out, and its inhibitory effect on the pathogenic bacteria of tomato bacterial wilt is shown in the photo figure 1 (The transparent blank circular area is the inhibitory effect of T-5 on the pathogenic bacteria of tomato bacterial wilt, and the milky white area is the pathogenic bacteria of bacterial wilt). The genus and species were identified, and the bacterial species were stored at -70°C in 30% glycerol by volume. The antagonistic bacteria T-5 was sent to the General Microorganism Center of China Microbiological Culture Collection Management Committee for preservation. The preservation date was December 06, 2013, and the preservation number was CGMCC NO.8547...

Embodiment 2

[0037] Embodiment 2 bacterial agent production

[0038]1) Carry out liquid fermentation culture on the Bacillus amyloliquefaciens strain T-5, and obtain the bacteria or spore content ≥ 4.0×10 9 The fermented liquid of each / ml, specifically:

[0039] Strain T-5 (CGMCC NO.8547, see Example 1) was inoculated into PDA culture fluid for liquid fermentation production. The fermentation conditions were as follows: the initial pH range was 7.0-7.2, the culture temperature was 30°C, and dissolved oxygen: The ventilation volume ranges from 30% to 100%, the rotation speed is 170 rpm, and spores are formed in the middle and late stages of fermentation. 9 pieces / ml.

[0040] The PDA culture medium configuration method used is (take the configuration of 1L medium as an example): Peel 200g of potatoes and cut into small pieces, put them into 500mL of water, heat and boil for 30min, add 20g of sucrose to the filtrate after filtration, and dilute to 1000mL , adjust the pH value to 7.0-7.4, ...

Embodiment 3

[0046] Embodiment 3 pot test

[0047] The soil used for the test was tomato continuous cropping soil, and bacterial wilt was serious. The tomato seeds are "Jiang Vegetable" which is sensitive to tomato bacterial wilt, and the treatment settings are as follows:

[0048] CK: control, tomato continuous cropping soil; T1: tomato continuous cropping soil + organic fertilizer treatment; T2: tomato continuous cropping soil + microbial organic fertilizer treatment

[0049] 5kg continuous cropping soil / pot, 30 pots per treatment, 3 seedlings / pot. T1: every pot uses 1% (W / W) organic fertilizer (50g, see the mixed organic fertilizer of step 2 in embodiment 3), T2: every pot uses 1% (W / W) microbial organic fertilizer (preparation method See embodiment 3), adopt hole application before transplanting seedlings, supplement N, P, K chemical fertilizer relative to 1% (W / W) organic fertilizer nutrient composition in the control treatment, all processings are equal nutrients.

[0050] The tom...

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Abstract

The invention relates to the field of microbial fertilizers, and particularly relates to a bacillus amyloliquefaciens strain T-5 for the prevention and treatment of tomato bacterial wilt and a microbial organic fertilizer thereof. The preservation number of the strain T-5 is CGMCC NO.8547. The T-5 containing living bacteria count of the strain T-5 containing microbial organic fertilizer disclosed by the invention is not less than 1.0*10<8> cfu / g. The invention also provides a method for preparing the strain T-5 containing microbial organic fertilizer. The strain T-5 disclosed by the invention is a specific efficient strain for inhibiting the growth of tomato bacterial wilt; the microbial organic fertilizer disclosed by the invention is rich in organic matters, amino acids and small molecule polypeptides, does not have a series of problems caused by the use of chemical pesticides, and both can be applied in feeding blocks and big fields; and the microbial organic fertilizer has the function of an organic fertilizer, and biocontrol bacteria contained in the microbial organic fertilizer are implemented through various growth promoting and biocontrol mechanisms, thereby achieving the effects of promoting growth and preventing and treating tomato bacterial wilt.

Description

technical field [0001] The invention relates to the field of microbial fertilizers, in particular to a Bacillus amyloliquefaciens strain for preventing and curing tomato bacterial wilt and microbial organic fertilizers thereof. Background technique [0002] Tomato (Lycopersicon esculentum Mill) is one of the main fruits and vegetables in the world. It occupies a very important position in human eating habits. It is widely planted in tropical, subtropical and temperate regions, and it is also widely planted in my country. Tomato bacterial wilt is a devastating soil-borne bacterial disease caused by Ralstonia solanacearum, which seriously restricts the development of tomato industry and the improvement of economic benefits. The disease is prone to outbreaks under high temperature and high humidity conditions, and is widely distributed in tropical, subtropical, and temperate regions. Ralstonia solanacearum is also found in some low-temperature regions, posing a great threat to ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C05F11/08A01P1/00C12R1/07
Inventor 谭石勇谭武贵丰来罗志威林炜
Owner 湖南慧谷农业生态研究院有限公司
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