Enrichment culture method of indigenous nitrogen transforming microorganism and application of same to treatment of water ammonia-nitrogen pollution

Abstract

The invention discloses an enrichment culture method of an indigenous nitrogen transforming microorganism and application of the same to the treatment of water ammonia-nitrogen pollution and belongs to the fields of environmental microbiology and environmental protection. An environmental sample is acquired from a water body required to be treated and cultured by a specific enrichment culture medium to obtain an enrichment culture seed of the indigenous nitrogen transforming microorganism. The enrichment culture seed and the enrichment culture medium are added into an in-situ water sample to carry out amplification culture, and then, the enrichment culture seed obtained through expansion culture is applied to the water body, so that the water ammonia-nitrogen pollution is treated. The efficient indigenous nitrogen transforming microorganism obtained through the method for carrying out enrichment amplification on the in-situ sample has better pertinence than a common commercial bactericide, and also avoids the adaptability of an external bactericide to the water body and potential ecological hazard. The rapid growth of the efficient indigenous nitrogen transforming microorganism is ensured through carrying out enrichment under manual controllable conditions, the effect is more stable than the effect of directly adding a growth promoter to the water body, and secondary pollution risks are avoided.

Description

technical field [0001] The invention belongs to the fields of environmental microbiology and environmental protection, and relates to a method for enriching and cultivating indigenous nitrogen-transforming microorganisms and its application in the treatment of ammonia-nitrogen pollution in water bodies. Background technique [0002] Industrial and agricultural production and life discharge a large amount of nutrients (such as nitrogen and phosphorus) to water bodies, resulting in eutrophication of water bodies. This is one of the most serious problems facing my country's water environment. [0003] Ammonia nitrogen is an important indicator for monitoring and controlling eutrophication. At present, the most stringent standard for urban sewage treatment plants in my country - the first-level A discharge standard, the discharge requirement for ammonia nitrogen is 5mg / L, while the standard for ammonia nitrogen in surface water IV in my country is 1.5mg / L. This means that with...

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Problems solved by technology

Although there are successful cases in the application of these bacterial agents, there are also problems that are difficult to overcome: these microbial agents are all foreign microorganisms in the environment, and there are problems of acclimatization to the soil and water, and their use effects are not very stable; even these microorganisms are also biologically invasive. Ecological security risk

Compared with the microbial agent technology, this technology has greatly reduced the risk of ecological safety, but it also has shortcomings: microbial growth promoters are likely to cause secondary pollution to the environment, and its growth-promoting effect is demanding on environmental conditions, so it is difficult to promote on a large scale. Therefore, its application is limited

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