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Fritillaria flower extractive, preparation method therefore and applications

A technology of extract and fritillary flower, which is applied in the field of medicine, can solve the problems of complex ingredients, low content, and lack of versatility, and achieve the effect of simple operation and reasonable process

Active Publication Date: 2015-08-26
ZHEJIANG CONBA PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method directly extracts water, contains a large amount of amino acids, and has high nutritional value, but the disadvantage is that the extraction rate of alkaloids is low, and there is a problem of loss of active ingredients.
[0011] To sum up, there are very few researches on the development of Fritillaria fritillaria at present. It has been disclosed that the composition of Fritillary fritillary flower extract is very complex, and the total alkaloids are usually used as the content control index, and its content is very low, and the clinical treatment effect is not obvious; And the extraction rate of alkaloid in the existing extraction method is low, does not have versatility

Method used

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  • Fritillaria flower extractive, preparation method therefore and applications
  • Fritillaria flower extractive, preparation method therefore and applications
  • Fritillaria flower extractive, preparation method therefore and applications

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Embodiment 1: peiminin A, peiminin B content assay method

[0032] Measured according to the "High Performance Liquid Chromatography Inspection Standard Operating Procedure" stipulated in the Pharmacopoeia.

[0033] Chromatographic conditions and system suitability test: use octadecylsilane bonded silica gel as filler; use acetonitrile as mobile phase A, 0.1% diethylamine as mobile phase B, carry out gradient elution as specified in the table below, evaporate light Scatter detector detection. The number of theoretical plates should not be less than 2000 based on the peak of peiminine A.

[0034] Table 1: Gradient elution conditions

[0035] time (min) Mobile phase A(%) Mobile phase B(%) 0-5 50 50 5-20 50-70 50-30 20-30 70-90 30-10

[0036] Preparation of reference substance solution: Take appropriate amounts of peiminin A reference substance and peiminin B reference substance, weigh them accurately, add methanol to make a mixed sol...

Embodiment 2

[0039] Example 2: Taking the content and transfer rate of peiminin A and peiminin B in the extract as an investigation index, the ethanol solution pH value, ethanol volume fraction, solid-liquid ratio, ultrasonic times, ultrasonic time, and relative density of thick paste and ethanol consumption.

[0040] 1. Take 10 g of Fritillaria flower powder, use 10 times the volume of 0.5% HCl, 0.05% HCl, 40% (v / v) ethanol solution of pH = 1, 2, 3, 4, 5, 6, ultrasonically extract 3 times , 1 hour each time, the filtrate was concentrated and dried, and the content of peiminin A + peiminin B in the extract and its transfer rate were determined.

[0041] The test results show that: when the pH is 3-4, the content and transfer rate of peiminin A + peiminin B in the extract are the best.

[0042] Table: 2: Influence of solvent acidity on extraction effect

[0043] 0.5% HCL 0.05% HCl pH=1 PH=2 pH=3 pH=4 pH=5 PH=6 content 0.326％ 0.417％ 0.469％ 0.619％ 1.694％...

Embodiment 3

[0070] Embodiment 3: purification process selection

[0071] Take the best extraction process to collect the filtrate and process it, then apply macroporous resin HPD100, HPD722, D101, neutral alumina column, Sephadex LH-20 dextran gel column, cation exchange resin column, collect effective parts, concentrate dry. Determination of the transfer rate and content of peimin A + peimin B.

[0072] The test results show that: Sephadex LH-20 Sephadex column has obvious advantages in transferring and enriching peimin A and peimin B.

[0073] Table 9: Comparison of extracts from different purification processes

[0074] Purification process HPD100 HPD722 D101 Neutral alumina LH-20 cation transfer rate 65.62％ 60.31％ 70.56％ 58.69％ 83.15％ 75.49％ content 16.67％ 14.64％ 19.78％ 10.12％ 46.59％ 13.58％

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Abstract

The invention provides a fritillaria flower extractive, a preparation method therefore and applications. The content of peimine and peiminine in the fritillaria flower extractive is 20%-60%(w / w) of the total extractive. The fritillaria flower extractive is used for preparation of medicines or health care products with cough relieving and phlegm resolving effects. The preparation method comprises ultrasonic extraction, filtrate condensation, alkalization, sephadex gel column separation and eluate condensation and drying, and the fritillaria flower extractive is obtained. The preparation method is simple to operate, effective components of fritillaria flowers can be collected effectively, and the fritillaria flower extractive has good development and application prospects.

Description

technical field [0001] The invention belongs to the technical field of medicine, and in particular relates to a fritillary flower extract, a preparation method and application thereof. Background technique [0002] The genus Fritillaria of the Liliaceae family includes a variety of important medicinal plants, and its medicinal parts are mostly underground bulbs. Fritillaria has the effects of clearing away heat and moistening the lungs, relieving cough and eliminating phlegm. According to records, there are about 130 species in this genus. In my country, its species mainly include Chuan Fritillaria, Zhejiang Fritillaria, Iberia Fritillaria, etc.; the research on the chemical constituents of Fritillaria is mainly focused on its alkaloid components, and there are relatively few literature reports on the non-alkaloid components of Fritillaria. At present, it has been found that the main active ingredients in Fritillaria are isosteroid alkaloids and steroidal alkaloids. Up to no...

Claims

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Application Information

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IPC IPC(8): A61K36/8966A61P11/10A61P11/14A61K133/00
Inventor 胡江宁何厚洪吴健周祥徐洪明楼芳芳瞿伟沈晓蓉
Owner ZHEJIANG CONBA PHARMA
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