Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of preparation method of simple theaflavin monomer

A technology of theaflavins and monomers, which is applied in the field of preparation of simple theaflavin monomers, can solve the problems of decreased physiological activity, precipitation, low solubility of ester-type theaflavins, etc., and achieves the effect of low adsorption rate

Active Publication Date: 2017-12-29
HUNAN AGRICULTURAL UNIV
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Aiming at the low solubility of ester-type theaflavins, which are prone to precipitate and decrease in physiological activity when added to black tea beverages, the present invention proposes a simple preparation method of theaflavins (TF), the specific steps of which are as follows:

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of preparation method of simple theaflavin monomer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] 1. Take 10 grams of 40% theaflavin extract, make 500ml theaflavin solution, add 80mg tannase and 20mg pectinase to the solution, stir at 25°C for 30 minutes for enzymolysis, and obtain enzyme Solution.

[0024] 2. Rapidly raise the temperature of the enzymolysis solution to 90°C and keep it for 5 minutes. After the enzymolysis solution cools down to room temperature, centrifuge at 8000r / min for 10min, take the supernatant and filter it.

[0025] 3. Slowly pass the supernatant filtered in step 2 through the chromatographic column equipped with DM130 adsorption resin, and elute with 10%, 30%, and 50% ethanol aqueous solution respectively, and collect 50% ethanol eluate, And the eluate was concentrated to 16-18 Baume degrees to obtain a concentrated solution.

[0026] 4. Put the concentrated solution at 0-4° C. for 48 hours, add a small amount of theaflavin monomer to crystallize the concentrated solution, then centrifuge and dry to obtain 2.13 g of simple theaflavins. A...

Embodiment 2

[0029] 1. Take 10 grams of 60% theaflavin extract and make 1000ml theaflavin solution, add 90mg tannase and 15mg pectinase to the solution, stir at 28°C for 40 minutes for enzymolysis to obtain enzyme Solution.

[0030] 2. Rapidly raise the temperature of the enzymolysis solution to 85° and keep it for 10 minutes. After the enzymolysis solution cools down to room temperature, centrifuge at 8000r / min for 15min, take the supernatant and filter it.

[0031] 3. The filtered supernatant in step 2 is passed through a chromatographic column equipped with D140 adsorption resin, and is eluted with 20%, 40%, and 60% ethanol aqueous solution respectively, and 60% ethanol eluent is collected, and the The eluent was concentrated to 16-18 degrees Baume to obtain a concentrated solution.

[0032] 4. Put the concentrated solution at 0-4° C. for 60 hours, add a small amount of theaflavin monomer to crystallize the concentrated solution, then centrifuge and dry to obtain 3.25 g of simple theaf...

Embodiment 3

[0034] 1. Take 10 grams of 80% theaflavin extract, make 1000ml theaflavin solution, add 100mg tannase and 20mg pectinase to the solution, stir at 30°C for 50 minutes for enzymolysis, and obtain enzyme Solution.

[0035] 2. Rapidly raise the temperature of the enzymolysis solution to 90° and keep it for 5 minutes. After the enzymolysis solution cools down to room temperature, centrifuge at 10000r / min for 20min, take the supernatant and filter it.

[0036] 3. Slowly pass the filtered supernatant in step 2 through the chromatographic column equipped with D140 adsorption resin, and elute with 20%, 40%, and 60% ethanol aqueous solution respectively, and collect 60% ethanol eluate, The eluent was concentrated to 16-18 degrees Baume to obtain a concentrated solution.

[0037] 4. Put the concentrated solution at a temperature of 0-4° C. for 72 hours, add a small amount of theaflavin monomer to crystallize the concentrated solution, then centrifuge and dry to obtain 4.39 g of simple t...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
concentrationaaaaaaaaaa
Login to View More

Abstract

The present invention relates to a method for preparing simple theaflavin monomer, which comprises the following steps: 1) adding a mixture of tannase and pectinase to a solution containing theaflavin extract, and heating the solution at 25°C-30°C Stir for 20-60min to obtain the enzymatic hydrolysis solution; 2) rapidly heat up the enzymatic hydrolysis solution to 85-90 ℃, keep for 5-10min, after the enzymatic hydrolysis solution is lowered to room temperature, centrifuge, and take the supernatant; pass the supernatant through Put it into a chromatographic column equipped with adsorption resin, and carry out fractional elution with ethanol eluent, collect the eluate, and concentrate the eluate to 16-18 Baume degrees to obtain a concentrated solution; It is placed under the temperature for 48 to 72 hours, a small amount of theaflavin monomer is crystallized, centrifuged and dried to obtain simple theaflavin (TF) monomer. The method of the invention uses compound enzymes to enzymatically hydrolyze theaflavins, has good conversion effect, and is a preparation method with high conversion rate and high product purity.

Description

technical field [0001] The invention belongs to the field of preparation of theaflavins, and in particular relates to a preparation method of simple theaflavins monomers. Background technique [0002] Theaflavins (Theaflavins, TFs) are a class of pigments formed by enzymatic oxidation and condensation of catechins during black tea processing, and are highly related to the quality of black tea. The mass fraction of theaflavins in black tea reaches 0.5%-2%. TFs is a general term for a class of compounds that are soluble in ethyl acetate and have a benzotropolone structure. Theaflavin (TF), Theaflavin-3-Gallate (TF-3-G), Theaflavin-3′-Gallate (TF-3′-G) and Theaflavin Digallate (TFDG) There are 4 main theaflavins. Among them, TF is called simple theaflavins because it does not contain galloyl groups in its chemical structure, and the latter three theaflavins contain one or two galloyl groups in their chemical structures and are called ester-type theaflavins. It has been confi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P17/16
Inventor 刘仲华王坤波黄建安林勇林海燕李娟
Owner HUNAN AGRICULTURAL UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com