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A lipid-lowering application of extract of trifoliate orange

An extract, lipid-lowering technology, applied in the directions of medical preparations containing active ingredients, drug combinations, food science, etc., to improve high-density lipoprotein cholesterol levels, reduce triglyceride and low-density lipoprotein cholesterol levels, and prepare low cost effect

Active Publication Date: 2017-12-05
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there are few reports on whether the extract of Hovenia trifoliata has lipid-lowering activity in vivo.

Method used

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  • A lipid-lowering application of extract of trifoliate orange
  • A lipid-lowering application of extract of trifoliate orange
  • A lipid-lowering application of extract of trifoliate orange

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0012] Example 1 Preparation of Hovenia trifoliate Extract

[0013] The mature fruit of Hovenia citrifolia (collected from Huangyan District, Taizhou City, Zhejiang Province) was manually separated to obtain the oil cell layer, white cortex layer, capsule coat and juice sac, and was quick-frozen in liquid nitrogen and then dried in a low-temperature vacuum, and the freeze-dried powder was obtained after grinding. Weigh 1 g of the freeze-dried powder of the oil cell layer, white cortex, capsule coat and juice sac, respectively, and use 20 mL of 80% methanol (solid-to-liquid ratio 1:20) to sonicate for 30 min at a frequency of 60 kHz and a power of 30 W. The extract was centrifuged at 10,000 rpm to obtain a clarified supernatant, which was extracted three times, the supernatants were combined, and methanol was evaporated on a rotary evaporator to obtain an aqueous solution of Hovenia trifoliate extract.

[0014] Aqueous solution with solid phase extraction Sep-Pak ® C18 column...

Embodiment 2

[0015] Example 2 C57BL / 6 mouse test of Hovenia trifoliate extract

[0016] C57BL / 6 mice were purchased from Shanghai Slack Experimental Animal Co., Ltd., and the low-fat diet (containing 10% fat) and high-fat diet (containing 45% fat) were purchased from Jiangsu Medison Biomedical Co., Ltd. All mice were raised in a constant temperature and humidity environment, providing 12 hours of light and 12 hours of darkness. 10 mice in each group, the low-fat+clear water group is the low-fat diet group, the high-fat+clear water group is the high-fat diet-induced obesity group, and four treatment groups are the oil cell layer, white cortex, capsule coat and juice sac extract Doses were all 40 mg / kg BW. All mice were free to ingest food and distilled water, the treatment group was administered by intragastric injection, and the low-fat and high-fat control groups were intragastrically administered distilled water. The test lasted for 13 weeks, with continuous administration for 6 days a...

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Abstract

The present invention provides the application of a trifoliate orange extract in the preparation of lipid-lowering functional food, health product or medicine. Extracts of citrus oil cell layer, white cortex, capsule coat and juice sac are obtained from their freeze-dried powder, ultrasonic extraction with methanol, rotary evaporation, solid phase extraction column, and vacuum drying. Hovenia citrus extract can significantly reduce body weight, significantly reduce liver, peritesticular fat, perirenal fat and brown fat weight, and significantly reduce serum triglyceride and low-density lipoprotein cholesterol levels in high-fat-induced obese C57BL / 6 mice , significantly increased serum HDL cholesterol levels. It can be used as functional food, health product or preventive medicine for the prevention and treatment of diseases related to abnormal fat metabolism.

Description

technical field [0001] The invention belongs to the field of food and medicine, and relates to the application of natural-sourced trifoliate orange extract in the preparation of lipid-lowering functional food, health products or medicines, in particular to the preparation method of the trifoliate orange extract and the application of the trifoliate orange extract in mice. Background technique [0002] Lipid is an important biological macromolecule to maintain the normal physiological function of cells and maintain the balance of the body. In modern society, due to changes in lifestyle, obesity, cardiovascular disease, diabetes and many other chronic non-communicable diseases caused by abnormal lipid and lipoprotein metabolism are on the rise. At present, there are many means of regulating abnormal lipid metabolism on the market. For example, statins target the cholesterol synthesis pathway, have strong cholesterol-lowering effects, and are important drugs for the treatment o...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K36/752A61P3/06A23L33/00
Inventor 李鲜贾胜孙崇德陈昆松
Owner ZHEJIANG UNIV
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