Traditional Chinese medicine toad venom dissimlar extract and preparation method thereof

Abstract

The invention relates to a traditional Chinese medicine toad venom dissimlar extract and a preparation method thereof, and belongs to the technical field of drugs. The preparation method of the extract comprises the steps of crushing toad venom medicinal materials into middle power, and adding 4 to 10 times of water for grinding and dispersing; adding ethyl alcohol to carry out alcohol precipitation, and storing at a low temperature; adding sodium chloride into supernatant liquor for salting out at a low temperature; carrying out concentration under reduced pressure until block-shaped toad venom dissimlar extract is separated out, carrying out drying or concentration under reduced pressure until nearly dry, carrying out vacuum drying, and adding ethanol recrystallization, so as to obtain the toad venom dissimlar extract containing resibufogenin, cinobufagin venom toad and bufalin; the transfer rate of the mixture of three bufogenins is about 80%, and the sum of weight percentages of the three bufogenins is equal to or greater than 35%. The toad venom extracted by adopting the method has the advantages of high transfer rate and active ingredient content, no residual organic solvent, simple and convenient operation, low energy consumption, and easiness in controlling of product quality, and is suitable for industrializing production.

Description

technical field [0001] The invention relates to a traditional Chinese medicine toad venom extract and a preparation method thereof, belonging to the technical field of medicines. Background technique [0002] Toad venom is a commonly used traditional Chinese medicine, which has been used as early as the Han Dynasty. It is recorded in "Shen Nong's Materia Medica" that "the taste is pungent and cold, governing the qi, breaking knots and hardening blood, carbuncles, genital sores, and taking it will not cause fever." Chinese medicine believes that toad venom Nature and flavor are pungent, warm; poisonous, Guixin Meridian, has detoxification, pain relieving, effect of resuscitating the mind. Modern studies have shown that toad venom has various pharmacological effects such as anti-tumor, cardiac strengthening, and blood pressure boosting. Especially in the field of cardiovascular and anti-tumor, the traditional Chinese medicine Bufotoxin has been widely studied and applied in r...

AI Technical Summary

Problems solved by technology

According to the first method of reflux extraction, although the operation is simple, the obtained extract has many impurities, and the content of active ingredients (based on resibufagenin, cinobufagin and bufafolin) is low, and it is difficult to meet the requirements of small drug loading. formulation requirements of dosage forms; although the second method can obtain components or monomers with relatively high content, the operation process is complicated, and the organic solvent may pollute the environment, and there will inevitably be organic solvent residues in the obtained monomers. It is difficult to ensure the synergistic effect of the ingredients of traditional Chinese medicine when it is prepared as a monomer

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