Microbial detection method based on immunomagnetic separation and urease catalysis

Abstract

The invention discloses a microbial detection method based on immunomagnetic separation and urease catalysis; target microorganisms are separated and enriched through immunomagnetic breads modified by biological recognition elements, and the concentration of the target microorganisms is improved; the salt ion concentration of a urea solution is changed by a urease catalytic reaction, so that a detection signal is effectively enlarged, and the detection sensitivity is substantially improved; moreover, the urease catalytic reaction belongs to a liquid-liquid phase reaction, the efficiency of the reaction mode is higher than that of a traditional solid-liquid phase reaction, the detection speed can be substantially improved, and a quantitative detection result of a small amount of microorganisms is obtained within 2 hours.

Description

technical field [0001] The invention relates to the field of biological detection, and more particularly to a microorganism detection method based on immunomagnetic separation and urease catalysis. Background technique [0002] In recent years, food safety accidents are in a period of frequent occurrence, and food safety has become one of the most prominent problems faced by our country. Among them, food safety incidents caused by pathogenic microorganisms have increasingly attracted the attention of the government and scientific researchers due to their characteristics of great harm and difficulty in prevention and control. Early screening of pathogenic microorganisms is the key to preventing and controlling such food safety incidents. Some conventional detection methods are still unable to take into account the high sensitivity and rapidity of detecting microorganisms, so there is an urgent need to develop a high-sensitivity and rapid detection technology for pathogenic mi...

AI Technical Summary

Problems solved by technology

At present, the impedance biosensor method is usually to modify the surface of the interdigital array microelectrode with biorecognition elements to specifically capture the target, and then measure the impedance change caused by the capture of the target to determine the content of the target. However, this method mainly There are two disadvantages: (1) the modification of biometric components on electrodes usually requires complex and lengthy biomodification processes, system repeatability is not ideal, and electrodes are difficult to reuse, which limits its practical application; (2) ) The capture of the target by the biorecognition element modified on the electrode surface is a solid-liquid phase reaction process, resulting in a low capture efficiency of the target, which greatly reduces the detection sensitivity of the system

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