A Microfluidic Cell Sorting System Driven by External Piezoelectric Ceramics

Abstract

The invention relates to a microfluid cell sorting system driven by external piezoelectric ceramic. The microfluid cell sorting system is characterized in that a metal spring for driving the sorting is encapsulated in a microfluid sorting chip, piezoelectric ceramic is arranged on the external side of the metal spring; a piezoelectric ceramic driven module converts a trigger signal into a high voltage signal for driving the piezoelectric ceramic, thus the piezoelectric ceramic will generate a mechanical displacement, the metal spring is extruded or released, and the target cells can be distributed to different sorting openings so as to sort the target cells. The driving of the microfluid sorting chip is controlled by the constant pressure valve of a sample injection module; an optical detection module carries out illumination detection on a single cell; a realtime signal control module analyzes the type of the single cell; the external piezoelectric ceramic drives the high-speed cell sorting; the whole system can carry out cell sample injection and separation under an extremely low pressure, the cells will not be damaged; the system is minimized and is capable of carrying out comprehensive analysis, has an very important meaning for promoting applications of regenerative medicine and tumor prognosis detection, and can be widely used for individual flow type cell sorting.

Description

technical field [0001] The invention relates to the technical field of biological research and clinical application, in particular to a microfluidic cell sorting system driven by an external piezoelectric ceramic. Background technique [0002] Flow Cytometer (FCM for short) is a high-tech instrument integrating optics, mechanics, electronics, and fluids for the analysis of fluorescent markers in biological cells. It is mainly used to measure and statistically analyze the fluorescence intensity information of various markers of a large number of stained cells. It is developed on the basis of single cell analysis and sorting. , RNA, protein, antigen, etc., are quickly measured and collected according to their characteristics. FCM is widely used in basic research and clinical practice of various biochemistry due to its fast, flexible, sensitive, quantifiable and large sample characteristics. Existing commercial flow cytometers can be divided into four parts: flow chamber and ...

AI Technical Summary

Problems solved by technology

Microfluidic sorting chips are usually bonded and bonded by plastic or polymer materials containing tiny channels, but existing microfluidic cell sorting schemes, such as electroosmotic flow, electrophoresis, pneumatic control, mechanical valves, optical tweezers, etc. Sorting schemes such as photothermogel and photothermal gel have the problems of slow sorting speed or complex structure and high cost; some high-throughput cell sorting methods such as dielectrophoresis, ultrasound, and surface acoustic wave separation methods rely on the characteristics of the cells themselves. Its universality is poor

In addition, most microfluidic chips use aqueous sheath fluid to focus on the cell position, resulting in a large dilution of the sorted sample, which is inconvenient for the detection of a small amount of samples

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