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Zhejiang anoectochilus roxburghii seed tissue culture and rapid seedling raising method

A technology of tissue culture and golden clematis, which is applied in horticultural methods, botany equipment and methods, horticulture, etc., can solve the problems of no stored nutrients found in seeds, no stored nutrients, and incomplete development of embryos, etc., to achieve a solution Seedling breeding problems, less investment, no effect of invalid seedlings

Active Publication Date: 2015-08-12
福建省农业科学院农业生物资源研究所
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Problems solved by technology

[0003] Since Zhejiang Golden Line Orchid is a plant of the Orchidaceae, the seed embryo is not fully developed, the germination rate of the seeds is extremely low under natural conditions, and the regeneration of resources is slow.
With the artificial excavation and the destruction of the ecological environment, the wild Zhejiang golden line orchid resources are on the verge of extinction. In addition, the seeds of Orchidaceae plants have a simple structure and hardly store nutrients, and no tissues that store nutrients have been found in the seeds. It is difficult to germinate under natural conditions, and the seedlings grow slowly

Method used

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  • Zhejiang anoectochilus roxburghii seed tissue culture and rapid seedling raising method
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  • Zhejiang anoectochilus roxburghii seed tissue culture and rapid seedling raising method

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specific Embodiment approach

[0024] A method for tissue culture and rapid seedling cultivation of Zhejiang Golden Thread Blue seeds, characterized in that: the method includes the following steps in sequence:

[0025] (1) Fruit selection: During the flowering period of Zhejiang Golden Thread Blue, select excellent Zhejiang Golden Thread Blue strains for artificial same or different plant pollination. 70-80 days after pollination, the selection is basically mature and the appearance color is close to yellow or The yellow-brown fruits are used for tissue culture; or the capsules on wild Zhejiang Golden Thread Blue plants collected in the field, choose the fruit that has a yellow or yellowish-brown appearance and is not cracked for tissue culture;

[0026] (2) Aseptic seeding and germination culture: the fruit selected in step (1) is soaked in alcohol with a mass percentage concentration of 70-85% for 30-45 seconds, and placed in a mercury solution with a mass percentage concentration of 0.08-0.15% Sterilize in m...

Embodiment 1

[0043] Example 1 (best example)

[0044] A method for tissue culture and rapid seedling cultivation of Zhejiang Golden Thread Blue seeds, characterized in that: the method includes the following steps in sequence:

[0045] (1) Fruit selection: During the flowering period of Zhejiang golden thread orchid, select excellent Zhejiang golden thread orchid strains for artificial same or different plant pollination. 75 days after pollination, the selection is basically mature and the appearance color is close to yellow or yellowish brown. The fruit is used for tissue culture;

[0046] (2) Aseptic seeding and germination culture: the fruits selected in step (1) are soaked in alcohol with a concentration of 75% by mass for 40 seconds, and then placed in a solution with a concentration of 0.1% by mass mercury for 10 minutes. After washing with sterile water for 4 times, cut the fruit, take out the white powdery or syrupy embryos and inoculate them on the seed germination medium. The seed germ...

Embodiment 2

[0056] A method for tissue culture and rapid seedling cultivation of Zhejiang Golden Thread Blue seeds, characterized in that: the method includes the following steps in sequence:

[0057] (1) Fruit selection: The full, hard, disease-free and light brown capsules collected from the wild Zhejiang Golden Thread in Jianyang, Fujian Province after natural pollination are used for tissue culture;

[0058] (2) Aseptic seeding and germination culture: The fruit selected in step (1) is soaked in alcohol with a mass percentage concentration of 80% for 45 seconds, and then placed in a 0.12% mercury solution with a mass percentage concentration for 12 minutes. After rinsing with sterile water for 3 times, cut the fruit and take out the white powdery or syrupy embryos and inoculate them on the seed germination medium. The seed germination medium is composed of the following components according to the following ratio: MS basic medium + 120g / L mashed potatoes+120ml / L coconut water+20g / L white s...

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Abstract

The invention relates to a Zhejiang anoectochilus roxburghii seed tissue culture and rapid seedling raising method, and belongs to the technical field of plant tissue culture and rapid propagation. The method comprises the following sequential steps: (1) fruit selection; (2) aseptic sowing, germination, and cultivation; (3) invigoration culture of aseptic seedling; (4) multiplication culture of cluster buds; (5) rooting and strengthening culture; (6) acclimatization and transplanting. The provided method is simple in operation, can obtain massive plants with excellent properties, can be applied to industrial production and inheritance, and has the advantages of low investment, and short production period (6 to 7 months). The seedling grows quickly and is very strong. The wild Zhejiang anoectochilus roxburghii resource can be effectively protected, and continuous production can be achieved.

Description

Technical field [0001] The invention relates to a method for seed tissue culture and rapid seedling breeding of Zhejiang golden thread orchid, belonging to the technical field of plant tissue culture rapid propagation. Background technique [0002] Zhejiang Golden Thread Orchid Anoectochilus zhejiangensis Z.Wei&Y.B.Chang, also known as Zhejiang Open-lip Orchid, is one of the rare and endangered orchid plants in my country and has been included in the list of national secondary protected plants and the list of rare and endangered plants in Zhejiang Province. The wild is distributed in Zhejiang, Fujian, Guangxi and other places, and was born in the damp place under dense forests at an altitude of 700-1200 meters on hillsides or valleys. It is a traditional and precious herbal medicine in my country, and it is widely used in folk. [0003] Because Zhejiang Golden Thread is a plant of the genus Orchidaceae, the embryo development is not complete, the germination rate of seeds under na...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈菁瑛刘保财
Owner 福建省农业科学院农业生物资源研究所
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