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Chiral sensor based on chitosan/cyclodextrin dinuclear copper and manufacturing method of chiral sensor

A cyclodextrin dual-nuclear copper and chitosan technology is applied in the field of electrochemical sensors and molecular recognition, which can solve the problems of long analysis time, high instrument cost, and difficulty in in-situ and online detection, and achieves high detection sensitivity and preparation. The method is simple and the effect of detecting the appropriate temperature

Inactive Publication Date: 2015-08-05
CHANGZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, this method has certain disadvantages, such as high instrument cost, long analysis time, and it is particularly difficult to realize in-situ and online detection, etc.

Method used

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  • Chiral sensor based on chitosan/cyclodextrin dinuclear copper and manufacturing method of chiral sensor
  • Chiral sensor based on chitosan/cyclodextrin dinuclear copper and manufacturing method of chiral sensor
  • Chiral sensor based on chitosan/cyclodextrin dinuclear copper and manufacturing method of chiral sensor

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] (1) Configure 5mM Cu 2 -β-CD solution (the solvent is 0.1M PBS, pH=7.0-9.0).

[0032] (2) Prepare CS solution (solvent is 0.1-0.3M HCl, pH=1.0-2.0), and deposit on the surface of glassy carbon electrode by constant potential to obtain non-alkali-treated CS / GCE modified electrode; place the electrode in alcohol The ammonia solution was subjected to alkali treatment for 15 minutes, then rinsed with a small amount of ethanol, and placed in air to dry to obtain an alkali-treated CS / GCE modified electrode.

[0033] (3) Place the electrode prepared in step 2 on the Cu prepared in step 1 2 -18~28h in β-CD solution, self-assembled to obtain CS / Cu 2 - β-CD modified electrodes.

Embodiment 2

[0035] In order to investigate CS / Cu 2 - Differences in the recognition ability of β-CD modified electrodes to different concentrations of Trp enantiomers.

[0036] Therefore, 0.05 mM, 0.2 mM, 0.3 mM, 0.4 mM, 0.5 mM, 0.6 mM, 0.8 mM, 1 mM, 2 mM, 5 mM of the Trp enantiomers were used, respectively. See the result image 3 , it can be seen that when the concentration of Trp enantiomer is 1mM, the oxidation peak current ratio of L / D-tryptophan (L / D-Trp) reaches the maximum.

Embodiment 3

[0038] In order to examine the different deposition times of CS, the CS / Cu 2-Differences in the ability of β-CD modified electrodes to recognize Trp enantiomers. Therefore, adopt the deposition time of 150s, 300s, 350s, 375s, 400s, 450s respectively, carry out the recognition of Trp enantiomer, its result sees Figure 4 , when the deposition time is 375s, CS / Cu 2 -The β-CD modified electrode has the best recognition effect on the Trp enantiomer. When the deposition time is too short, the amount of chitosan deposited on the surface of the glassy carbon electrode is relatively small, so that the quality of the formed chitosan film reaches Less than the desired experimental effect. When the deposition time is too long, the amount of chitosan adsorbed on the surface of the electrode will be too much, so that the thickness of the formed chitosan film will be thickened, which is not conducive to Cu 2 -Self-assembly of β-CD, affecting Cu 2 -β-CD interacts with chitosan, thereby a...

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Abstract

The invention relates to a chiral sensor based on chitosan / cyclodextrin dinuclear copper and a manufacturing method of the chiral sensor. Chitosan (CS) constant potentials are deposited on the surface of a glassy carbon electrode (GCE), a modified electrode is subjected to alkali treatment, and cyclodextrin dinuclear copper (Cu2-beta-CD) is self-assembled to obtain a novel sensor of CS / Cu2-beta-CD, and tryptophan enantiomers are selectively identified by a DPV (differential pule voltammetry) method. The manufacturing method includes steps of preparing Cu2-beta-CD, preparing modified GCE of alkali-treatment CS and CS / Cu2-beta-CD electrode, and electrochemically identifying L / D-Trp. The chiral sensor has the advantages that the CS / Cu2-beta-CD modified electrode is simple to operate, time saving and free of pollution. The result indicates that the glassy carbon electrode modified by the CS / Cu2-beta-CD has efficient identification effect for tryptophan enantiomer, and oxidation peak current ratio of L / D-tryptophan (L / D-Trp) is averagely up to 7.26.

Description

technical field [0001] Chitosan (CS) was deposited on the surface of the glassy carbon electrode at a constant potential, and the modified electrode was treated with alkali, and the self-assembled cyclodextrin binuclear copper (Cu 2 -β-CD), to obtain CS / Cu 2 - A novel sensor for β-CD, using differential pulse voltammetry (DPV) for selective recognition of tryptophan enantiomers, belonging to the field of electrochemical sensors and molecular recognition. Background technique [0002] Living systems contain a large number of biomacromolecules, most of which are enantioselective. Chirality is a common phenomenon in nature, such as sugars, amino acids, proteins and DNA are chiral molecules. Due to the similar physical and chemical properties of chiral compounds, it is difficult to distinguish them. Therefore, chiral recognition plays a crucial role in distinguishing chiral molecules. At present, chiral recognition research methods mainly include chiral chromatography, spect...

Claims

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Application Information

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IPC IPC(8): G01N27/30G01N27/48
Inventor 陶永新顾晓刚孔泳邹平
Owner CHANGZHOU UNIV
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