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Chiral sensor based on copper ion modified cyclodextrin/polyarginine/carbon nanotube and its preparation method

A technology of carbon nanotubes and polyarginine, which is applied in the field of electrochemical sensors and molecular recognition, can solve the problem of small effect and achieve the effect of simple operation, high detection sensitivity and easy availability of raw materials

Active Publication Date: 2020-08-04
SHANXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Especially arginine, its molecule has two amino groups, and forms a porous conductive polymer after electropolymerization, while other amino acid molecules carry only one amino group, so the effect is minimal

Method used

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  • Chiral sensor based on copper ion modified cyclodextrin/polyarginine/carbon nanotube and its preparation method
  • Chiral sensor based on copper ion modified cyclodextrin/polyarginine/carbon nanotube and its preparation method
  • Chiral sensor based on copper ion modified cyclodextrin/polyarginine/carbon nanotube and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Embodiment 1: A kind of chiral sensor based on copper ion modified cyclodextrin / polyarginine / carbon nanotube, described sensor is that arginine L-Arg is polymerized on the surface of carbon nanotube MWCNTs by electropolymerization method , and then placed in the copper ion modified cyclodextrin Cu-β-CD solution to prepare the Cu-β-CD / PLA / MWCNTs sensor. Prepare Cu-β-CD / PLA / MWCNTs sensor, the specific steps are as follows:

[0022] (1) Prepare 5mM Cu-β-CD solution (solvent is 0.1M PBS, pH=6.5~7.5).

[0023] (2) Prepare carbon nanotube (MWCNTs) dispersion (solvent is N, N-dimethylformamide (DMF)), drop the dispersion on the surface of the electrode, dry it under infrared light, and finally rinse it off with secondary water Loosely adsorbed MWCNTs to obtain MWCNTs modified electrodes.

[0024] (3) Put the electrode prepared in step 2 in the L-Arg solution (the solvent is 0.1M PBS, pH=6.0-7.0), and electropolymerize on the surface of the electrode to obtain a PLA / MWCNTs mo...

Embodiment 2

[0026] Example 2: In order to investigate the differences in the ability of Cu-β-CD / PLA / MWCNTs sensors to recognize Trp enantiomers at different concentrations of MWCNTs.

[0027] Therefore, 0.2 mg / mL, 0.4 mg / mL, 0.6 mg / mL, 0.8 mg / mL, and 1.0 mg / mL MWCNTs were used to modify the electrodes, respectively. See the result Figure 4 , it can be seen that when the concentration of MWCNTs is 0.6 mg / mL, the difference of the oxidation peak current of L / D-tryptophan (L / D-Trp) reaches the maximum.

Embodiment 3

[0028] Example 3: In order to investigate the differences in the ability of Cu-β-CD / PLA / MWCNTs modified electrodes to recognize Trp enantiomers at different polymerization cycles.

[0029] Therefore, the electrodes were modified with L-Arg that polymerized 5, 10, 15, 20, 25 circles. See the result Figure 5 , it can be seen that when L-Arg is polymerized for 20 cycles, the difference in the oxidation peak current of L / D-tryptophan (L / D-Trp) reaches the maximum. After 20 cycles, the current difference decreased slightly, which may be due to the basic saturation of the enantioselective reaction sites of PLA / MWCNTs.

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Abstract

The invention belongs to the technical field of electrochemical sensors and molecular recognition and provides a chiral sensor based on copper ion modified cyclodextrin / poly arginine / carbon nanotube and a preparation method thereof. The sensor is simpler in an electrode modifying process and has good stability and reproducibility. A tryptophan enantiomer can be selectively recognized efficiently by means of differential pulse voltammetry. Arginine L-Arg is polymerized to the surface of the carboxylated carbon nanotube by means of an electric polymerization method, and is then placed in a Cu-beta-CD solution to prepare the Cu-beta-CD / PLA / MWCNTs sensor. The tryptophan enantiomer can be selectively recognized efficiently by means of differential pulse voltammetry. The raw materials are simpleto prepare and are easily available, and the electrode is prepared in a time-saving manner free of pollution. PLA and Cu-beta-CD are combined through a Cu-N coordinate band to exert a synergistic effect to recognize the tryptophan enantiomer efficiently. The differential pulse oxidization peak current ratio of L / D-tryptophan can reach 3.37.

Description

technical field [0001] The invention belongs to the technical field of electrochemical sensors and molecular recognition, and relates to the preparation technology of electrochemical electrode materials, in particular to a chiral sensor based on copper ion modified cyclodextrin / polyarginine / carbon nanotubes and a preparation method thereof. Selective identification of tryptophan enantiomers was performed using differential pulse voltammetry (DPV). Background technique [0002] Living systems contain a large number of biomacromolecules, most of which are enantioselective. Chirality is a common phenomenon in nature, such as sugars, amino acids, proteins and DNA are chiral molecules. Due to the similar physical and chemical properties of chiral compounds, it is difficult to distinguish them. Therefore, chiral recognition plays a crucial role in distinguishing chiral molecules. At present, chiral recognition technologies mainly include high performance liquid chromatography, ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/327G01N27/48
CPCG01N27/308G01N27/3278G01N27/48
Inventor 雷鹏周影洪沙沙杨亚娟双少敏
Owner SHANXI UNIV
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