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Preparation method of high-purity humulus lupulus total flavones

A technology of hops and total flavonoids, which is applied in the field of preparation of high-purity hops total flavonoids, can solve problems such as insufficient supply, and achieve the effects of good product color, high product yield and content, and low cost

Active Publication Date: 2015-08-05
JIANGSU TIANSHENG PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] According to the situation of the above literature and patents, the current research is not enough to provide a preparation process suitable for the requirements of large-scale industrial production, which is used for extraction and purification, and finally obtains total flavonoids of hops with high content and good appearance.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] First, add 2kg of raw hops (hop ear) into 20L of 60% ethanol extract, reflux at 65°C for 2h, then filter and wash to obtain 19.6L of extract; Continue to reflux for 2 hours in the concentrated ethanol extract, filter, and combine the two extracts, which is about 41.0 L. Concentrate the obtained extract until it has no alcohol smell, then dilute it with water and let it stand to cool, then filter it through diatomaceous earth, pass the filtrate through a polyamide column, and wash it with purified water and ethanol eluent with a concentration of 70% in turn Remove, and collect the effluent of the eluted part of the ethanol eluent. Dilute the effluent with water and pass it through a DM130 macroporous resin column, elute again with purified water, 25% ethanol eluent and 95% ethanol eluent, and collect 95% ethanol eluent again The effluent from the liquid elution fraction. Finally, the effluent was decolorized by adding activated carbon, then suction filtered while it wa...

Embodiment 2

[0025] First, add 2kg of raw hops (hop ear) into 20L of ethanol extract with a concentration of 90%, reflux at 80°C for 2h, then filter and wash to obtain 18.2L of extract; Continue to reflux in the ethanol extract of equal concentration for 2 hours, repeat the extraction twice, filter, and combine the extracts three times, which is about 62.5 L. Concentrate the obtained extract until it has no alcohol smell, then dilute it with water and let it stand to cool, then filter it through diatomaceous earth, pass the filtrate through a polyamide column, and wash it with purified water and ethanol eluent with a concentration of 70% in turn Remove, and collect the effluent of the eluted part of the ethanol eluent. Dilute the effluent with water and pass through the HPD-600 macroporous resin column, elute again with purified water, 25% ethanol eluent and 95% ethanol eluent, and collect 95% ethanol again Eluent The effluent from the eluted fraction. Finally, the effluent was decoloriz...

Embodiment 3

[0027] Firstly, add 2kg of raw hops (hop ear) into 20L of ethanol extract with a concentration of 90%, reflux at 65°C for 2 hours, then filter and wash to obtain 20.3 L of extract; Continue to reflux in the ethanol extract of equal concentration for 2 hours, filter, and combine the two extracts, approximately 42.2 L. Concentrate the obtained extract until it has no alcohol smell, then dilute it with water and let it stand to cool, then filter it through diatomaceous earth, pass the filtrate through a polyamide column, and wash it with purified water and ethanol eluent with a concentration of 70% in turn Remove, and collect the effluent of the eluted part of the ethanol eluent. Dilute the effluent with water and pass it through a D101 macroporous resin column, elute again with purified water, 25% ethanol eluent and 95% ethanol eluent, and collect 95% ethanol eluent again The effluent from the liquid elution fraction. Finally, the effluent was added to activated carbon for dec...

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Abstract

The invention discloses a preparation method of high-purity humulus lupulus total flavones, wherein the preparation method includes following steps: (A) performing ethanol reflux to a raw material, humulus lupulus, to obtain an extract; (2) treating the extract through a polyamide column and passing the extract through a macroporous resin column, and performing elution to obtain a solution; (3) decoloring the solution through activated charcoal and spray-drying the solution to finally obtain the humulus lupulus total flavones being high in content and good in appearances. The preparation method is simple in steps, is less in usage of organic solvents during the process, is low in cost and is safe in operation and free of environment pollution. The final product is good in appearances and is high in yield and content. The preparation method is suitable for industrial production, fully utilizes two different ways of the humulus lupulus for brewing beer and making drugs, and provides new approaches of maximum utilization of the humulus lupulus.

Description

technical field [0001] The invention relates to a preparation method of high-purity hop total flavonoids, which belongs to the technical field of medicinal active ingredient extraction. Background technique [0002] Hops, called hops in "Compendium of Materia Medica", is a perennial herbaceous vine plant. The ancients took it as a medicinal material. It is native to Europe, America and Asia, and is distributed in northern Xinjiang, northeast China, North China, Shandong, Gansu, Shaanxi and other places. . The ears of hops are used for making beer, which can impart bitterness and aroma to beer, and hops can also be widely used as a plant at home and abroad. Hops have the effects of invigorating the stomach and eliminating food, antibacterial and anti-inflammatory, calming the nerves and diuresis, and anti-tuberculosis. It can be used to treat indigestion, edema, insomnia, dysentery, and tuberculosis. Studies have shown that hops mainly contain resins, volatile oils, flavono...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/60
Inventor 季浩张宇
Owner JIANGSU TIANSHENG PHARMA
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