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Method for purifying and separating gamma-polyglutamic acid from fermentation liquor

A polyglutamic acid, separation and purification technology, applied in the field of separation and purification of γ-polyglutamic acid, can solve the problems of high loss of organic solvent, high production cost products, organic solvent residue, etc., to achieve no secondary pollution, The effect of low production cost and no organic solvent residue

Active Publication Date: 2015-07-29
SHANDONG FREDA BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The present invention is characterized by the extraction process of controlling the pH value to precipitate and precipitate gamma-polyglutamic acid, and solves the problems of high organic solvent loss and long process caused by the organic solvent precipitation method for extracting gamma-polyglutamic acid. The product has the problem of organic solvent residue

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] (1) Fermentation broth pretreatment: heat the fermentation broth to 90°C, keep it warm for 1 hour, adjust the pH to 4.0 after cooling down to 60°C, remove the bacteria through plate and frame filtration, then adjust the pH to 5.0, heat to 80°C, add Activated carbon is decolorized by heat preservation for 1 hour. After the decolorization is completed, the plate and frame are filtered to remove the activated carbon to obtain a clear filtrate;

[0022] (2) Precipitation: adjust the pH of the filtrate to 2.5 with hydrochloric acid, let it stand for 24 hours to precipitate, filter the plate and frame to obtain a white powder, and wash it with purified water of pH 2.5 until the waste water is colorless and clear;

[0023] (3) Add the white powder into purified water, adjust the pH to 4.0, stir and dissolve to obtain a solution with a concentration of 30%;

[0024] (4) Then go through belt drying (the process conditions of belt drying are that the temperature in the heating zo...

Embodiment 2

[0026] (1) Fermentation broth pretreatment: heat the fermentation broth to 75°C, keep it warm for 2 hours, adjust the pH to 3.5 after cooling down to 60°C, remove the bacteria through plate and frame filtration, then adjust the pH to 6.0, heat to 85°C, add Activated carbon is decolorized by heat preservation for 0.5h. After the decolorization is completed, the plate and frame filter the activated carbon to obtain the clear filtrate;

[0027] (2) Precipitation: Adjust the pH of the filtrate to 3.0 with hydrochloric acid, let it stand for 12 hours, and precipitate the precipitate. Filter the plate and frame to obtain a white powder, and wash it with purified water with a pH of 3.0 until the waste water is colorless and clear;

[0028] (3) Add the white powder into purified water, adjust the pH to 4.5, stir and dissolve to obtain a solution with a concentration of 40%;

[0029] (4) Then go through belt drying (the process conditions of belt drying are that the temperature in the ...

Embodiment 3

[0031] (1) Fermentation broth pretreatment: heat the fermentation broth to 80°C, keep it warm for 1.5h, then adjust the pH to 4.0 after cooling down to 60°C, filter through the plate frame to remove bacteria, then adjust the pH to 6.0, heat to 85°C, Add activated carbon and keep it warm for 0.5h for decolorization. After the decolorization is completed, the plate and frame filter to remove the activated carbon to obtain the clear filtrate;

[0032] (2) Precipitation: Adjust the pH of the filtrate to 2.5 with hydrochloric acid, let it stand for 18 hours, and precipitate the precipitate. Filter the plate and frame to obtain a white powder, and wash it with purified water with a pH of 2.5 until the waste water is colorless and clear;

[0033] (3) Add the white powder into purified water, adjust the pH to 4.5, stir and dissolve to obtain a solution with a concentration of 35%;

[0034] (4) Then go through belt drying (the process conditions of belt drying are that the temperature ...

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Abstract

The invention discloses a method for purifying and separating gamma-polyglutamic acid from fermentation liquor. Through the utilization of the characteristic that only the gamma-polyglutamic acid can be separated out in a fermentation liquor filtrate with low pH value, the purpose of separating and purifying high-purity gamma-polyglutamic acid is achieved. The method comprises the following steps: adjusting the pH value to be 3.0-6.0 with acid, performing filtration sterilization, then, decolorizing with activated carbon, adjusting the pH value to be 2.0-5.0 with acid, leaving to stand, separating out sediments, performing solid-liquid separation, dissolving the sediments in purified water to obtain a high-concentration water solution, finally, performing band drying, and smashing, so as to obtain the gamma-polyglutamic acid finished product. According to the invention, the process route is novel and simple, an organic solve is completely avoided, the product has no organic solvent residue, the production cost is low, the cost is reduced by 30% or above, the yield is as high as 85% or above, the product quality is excellent, the purity reaches 95% or above, and the method is more suitable for industrial production.

Description

technical field [0001] The invention relates to a method for separating and purifying gamma-polyglutamic acid from fermentation liquid, belonging to the technical field of industrial microorganisms. Background technique [0002] γ-polyglutamic acid (γ-PGA) is a high-molecular amino acid polymer synthesized by microorganisms. It has a large number of highly active free side chain carboxyl groups (-COOH) on its molecular chain, and has extremely high water absorption and moisturizing properties. It is easy to combine with some drugs to form a stable complex, which is an ideal class of biodegradable medical polymer materials. It has no pollution to the environment, is a green biological product, and has many unique physical, chemical and biological properties such as excellent biodegradability, film-forming property, fibrillation property, plasticity, cohesiveness, moisture retention and many others. Today, with emphasis on environmental protection and sustainable development,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08G69/10
Inventor 张林军苏移山马双双朱云峰宗工理李珍爱刘英梅赵文刚
Owner SHANDONG FREDA BIOTECH
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