Method for breeding epsilon-poly-L-lysine tolerant high-producing strain

Abstract

The invention belongs to the field of microbial breeding and relates to a method for screening an epsilon-poly-L-lysine tolerant high-producing strain through restructuring a genome. The method is characterized in that 16g / L epsilon-poly-L-lysine is added in a restructured strain regeneration medium by using a 4-5 cycle progressive protoplast fusion method, and finally, the yield-increased restructured strain capable of growing at high concentration is obtained.

Description

technical field [0001] The invention belongs to the field of microbial breeding, and relates to a method for genome shuffling and screening of ε-polylysine-tolerant high-yield strains. Background technique [0002] ε-polylysine (ε-poly-l-lysine, ε-PL) is a microbial secondary metabolite formed by linking α-carboxyl and ε-amino groups in lysine monomers. Compared with chemically synthesized α-poly-l-lysine (α-poly-l-lysine, α-PL), ε-PL has the advantages of wider antibacterial spectrum, good biocompatibility and non-toxic and harmless. As a food preservative, it has been approved for use in Japan, the United States, South Korea and China, and the demand is increasing day by day. In addition, as a gene carrier and cell culture material, it can inhibit the activity of pancreatic lipase in the small intestine, hinder the absorption of fat in the diet, and reduce obesity and other fields of medicine and biotechnology. It has potential application prospects. [0003] However, ac...

AI Technical Summary

Problems solved by technology

[0004] Mutants of strains can be obtained by classical chemical mutagenesis, but this method usually requires a lot of time and effort

Although genetic engineering methods can change bacterial strains in a targeted manner, the prerequisite is that the genetic background of the relevant bacterial strains must be clear