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Preparation method of polymer/keratin composite anticoagulation vascular tissue engineering scaffold

A technology of vascular tissue and keratin, applied in anticoagulant treatment, types of packaging items, special packaging items, etc., can solve problems such as inability to meet the supply demand of small-diameter vascular grafts, low long-term patency rate, and thrombus formation , to achieve the effects of inhibiting the growth of smooth muscle cells, good cell compatibility, and reducing vascular restenosis

Active Publication Date: 2015-07-22
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In practice, artificial synthetic blood vessels are likely to cause thrombosis, intimal hyperplasia, and low long-term patency rate after implantation; autologous blood vessels are limited by the source, diameter and length of the donor, and cannot meet the supply of small-diameter vascular grafts need

Method used

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  • Preparation method of polymer/keratin composite anticoagulation vascular tissue engineering scaffold
  • Preparation method of polymer/keratin composite anticoagulation vascular tissue engineering scaffold
  • Preparation method of polymer/keratin composite anticoagulation vascular tissue engineering scaffold

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] The preparation method of the polymer / keratin composite anticoagulant blood vessel tissue engineering scaffold comprises the following steps in sequence:

[0028] ① Keratin reduction and stabilization

[0029] Human hair is washed sequentially with alkali and methanol to remove oil stains. Cut the crude human hair (1g), put it into a flask, add mercaptoethanol (3g), urea (20g), sodium dodecylsulfonate (1.5g), swell for 1-2 hours, heat to 50°C, and react 12h. After dialysis for 2 days, the keratin solution was obtained by filtration. Measure the sulfhydryl content of keratin after dialysis, calculate the total amount of sulfhydryl according to the volume, and add an excess modifier (iodoacetic acid) to stabilize the sulfhydryl, react in the dark for 12 hours at room temperature, dialyze again for 3 days, and freeze-dry to obtain modified After the keratin.

[0030] ②Construction of vascular tissue engineering scaffold by electrospinning

[0031] The polymer PCL is b...

Embodiment 2～3

[0036] The polymer / keratin composite anticoagulant vascular tissue engineering scaffold was prepared in the same manner as in Example 1. In step ② "electrospinning to construct a vascular tissue engineering scaffold", in the blend of polymer PCL and keratin, The mass percentages of keratin are 20% and 30% respectively, and other conditions are the same.

[0037] Fibroblasts and vascular endothelial cells were selected for culture, and cell compatibility was tested by MTT and other methods; the NO release ability was measured by simulating the blood environment, and the results are shown in image 3 and Figure 4 (9 / 1 among the figure represents PCL / keratin mass ratio); Adopt conventional coagulation testing method to measure anticoagulant performance.

[0038] The results showed that the prepared scaffolds had good cytocompatibility and anticoagulant properties, and could catalyze or induce the release of NO from endogenous NO donors in situ.

Embodiment 4

[0040] The preparation method of the polymer / keratin composite anticoagulant blood vessel tissue engineering scaffold comprises the following steps in sequence:

[0041] ① Keratin reduction and stabilization

[0042] Human hair is washed sequentially with alkali and methanol to remove oil stains. Shred the crude product (1g), put it into a flask, add mercaptoethanol (4g), urea (15g), sodium dodecylsulfonate (1.5g), heat to 60°C, and react for 24h. After dialysis for 1 day, the keratin solution was obtained by filtration. Measure the sulfhydryl content of keratin after dialysis, calculate the total amount of sulfhydryl according to the volume, and add excess modifier iodoacetic acid to stabilize the sulfhydryl, react in the dark at room temperature for 12 hours, dialyze again for 2 days, and freeze-dry to obtain the modified of keratin.

[0043] ②Construction of vascular tissue engineering scaffold by electrospinning

[0044] The polymer polyurethane is blended with keratin...

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Abstract

The invention discloses a method for constructing an anticoagulation vascular tissue engineering scaffold by utilizing keratin. The method for constructing the anticoagulation vascular tissue engineering scaffold by utilizing keratin comprises the following steps: extracting keratin, stabilizing the extracted keratin, and carrying out blended spinning on the extracted keratin and a biodegradable polymer to prepare a composite fiber diaphragm. The prepared composite fiber diaphragm is taken as a vascular tissue engineering scaffold, and NO release is realized by utilizing keratin having a capability of in situ catalyzing or inducing an endogenous NO donor to release No; and further blood compatibility and cytocompatibility of the scaffold are improved, growth of smooth muscles is inhibited, and finally in-situ vascularization is realized. The method can be used for preparing the vascular tissue engineering scaffold and also can be used for preparing other medical devices in contact with blood.

Description

technical field [0001] The invention relates to the preparation of a vascular tissue engineering scaffold material, in particular to a preparation method of a polymer / keratin anticoagulant vascular tissue engineering scaffold. Background technique [0002] Artificial blood vessels and autologous blood vessels are the main sources of small-diameter (<6mm) vascular grafts in clinical practice. In practice, artificial synthetic blood vessels are likely to cause thrombosis, intimal hyperplasia, and low long-term patency rate after implantation; autologous blood vessels are limited by the source, diameter and length of the donor, and cannot meet the supply of small-diameter vascular grafts need. Tissue-engineered blood vessels provide an effective way to solve the source of small-diameter vascular grafts. Preparation of scaffolds for cells to carry out life activities and biological evaluation of scaffold materials are important areas of tissue-engineered blood vessel researc...

Claims

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Application Information

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IPC IPC(8): A61L27/48A61L33/12A61L27/22A61L27/58A61L27/50
Inventor 袁江李鹏飞沈健
Owner NANJING NORMAL UNIVERSITY
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