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A galium verum extract product with estrogen activity, a preparing method thereof and applications of the extract product

A kind of technology of the extract of fennel, which is applied in the direction of medical preparations containing active ingredients, diseases, and drug combinations, etc., can solve the problems of flavonoid glycosides damage and other problems, and achieve weight gain, estrogen levels, and production. high rate effect

Inactive Publication Date: 2015-07-22
HEILONGJIANG UNIV OF CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the extraction of the total flavonoids of Potassia japonica has been reported to use water extraction followed by alcohol extraction, alkali-soluble acid precipitation, and organic solvent extraction and then resin column extraction. Although the total flavonoid content is high, the flavonoid glycosides It is easy to be hydrolyzed and destroyed in the process of alkali-soluble acid precipitation, and the introduction of a large amount of organic solvent is also one of the unfavorable factors

Method used

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  • A galium verum extract product with estrogen activity, a preparing method thereof and applications of the extract product
  • A galium verum extract product with estrogen activity, a preparing method thereof and applications of the extract product
  • A galium verum extract product with estrogen activity, a preparing method thereof and applications of the extract product

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Get 1kg of caperia coarse powder, first use 60% (v / v) ethanol to sonicate for 20min to fully break the cells, then use 60% (v / v) ethanol to reflux and extract twice, and the amount of ethanol used for the first reflux extraction is 10 Double the weight of the whole herb powder of A. japonica, the second reflux extraction of the whole herb meal of 8 times the weight of ethanol, the reflux extraction time is 1 hour each time. Filtrate while hot, combine the filtrates, and recover ethanol under reduced pressure at 50°C to the extract. AB-8 macroporous resin was routinely processed according to the instruction manual, and packed into a column. The amount of resin used was 2kg. After the resin column was eluted with 8% (v / v) ethanol for 2BV, the above extract was dissolved with 8% (v / v) ethanol, put on the resin column, and left to stand for 2h. First elute with 15% (v / v) ethanol at a flow rate of 1.0BV / h, elute 2BV, and remove impurities. Continue to elute with 50% (v / v) ...

Embodiment 2

[0027] Get 2kg of capsicum coarse powder, first ultrasonic 30min with 70% (v / v) ethanol, cell is fully broken, then reflux extraction twice with 70% (v / v) ethanol, the ethanol consumption of reflux extraction for the first time is 10 times the weight of the whole herb powder of A. japonica, and the second reflux extraction of the whole herb meal of 8 times the weight of ethanol, and the reflux extraction time is 1.5 hours each time. Filtrate while hot, combine the filtrates, and recover ethanol under reduced pressure at 50°C to the extract. AB-8 macroporous resin was routinely processed according to the instruction manual, and packed into a column, and the amount of resin was 6kg. After the resin column was eluted with 10% (v / v) ethanol for 2BV, the above extract was dissolved with 10% (v / v) ethanol, put on the resin column, and left standing for 3h. First elute with 20% (v / v) ethanol at a flow rate of 1.5BV / h, elute 2BV, and remove impurities. Continue to elute with 60% (v / ...

Embodiment 3

[0029] Get 3 kg of capsicum coarse powder, first use 80% (v / v) ethanol to sonicate for 40 minutes to fully break the cells, then use 80% (v / v) ethanol to reflux and extract twice, and the ethanol consumption for the first reflux extraction is 10 Double the weight of the whole herb powder of A. japonica, and the second reflux extraction of the whole herb meal of 8 times the weight of ethanol, the reflux extraction time is 2 hours each time. Filtrate while hot, combine the filtrates, and recover ethanol under reduced pressure at 50°C to the extract. AB-8 macroporous resin was routinely processed according to the instruction manual, and packed into a column. The amount of resin used was 12kg. After the resin column was eluted with 12% (v / v) ethanol for 2BV, the above extract was dissolved with 12% (v / v) ethanol, put on the resin column, and left standing for 4h. First elute with 25% (v / v) ethanol at a flow rate of 1.2BV / h, elute 2BV, and remove impurities. Continue to elute wit...

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Abstract

The invention discloses a galium verum extract product with estrogen activity, a preparing method thereof and applications of the extract product, and belongs to the technical field of Chinese herbal medicines. The method includes following steps: (1) subjecting whole herb coarse powder of galium verum to ultrasonic wave extraction and reflux extraction by using ethanol having a concentration of 60-80%, filtering the obtained extract liquid, and concentrating the extract liquid under vacuum to recover the ethanol to obtain an extract; and (2) dissolving the extract with ethanol having a concentration of 8-12%, adding into a macroporous adsorption resin column, allowing the column to stand, washing by ethanol having a concentration of 15-25% firstly to remove impurities, eluting with ethanol having a concentration of 50-70%, collecting ethanol eluate, concentrating the ethanol eluate under vacuum to recover the ethanol, and drying at a low temperature. The preparing method has advantages of simple processes, short period, high yield, and the like. The prepared galium verum extract product mainly comprises total flavonoid compounds. The content of the total flavonoid compounds is higher than 57%. The total flavonoid compounds mainly comprise luteoloside. The prepared galium verum extract product can stimulate mouse uterus growth, promote weight gain and increase the estrogen level.

Description

technical field [0001] The present invention relates to an extract of active ingredients of Chinese herbal medicines, in particular to an extract of Potassium officinalis with estrogen activity and a preparation method thereof. The application in medicine belongs to the technical field of Chinese herbal medicine extraction. Background technique [0002] The occurrence of many diseases is closely related to the level of estrogen in the body, such as osteoporosis, cardiovascular disease, etc. As women enter menopause, the risk of these diseases also increases significantly. Although the replacement therapy of exogenous hormone supplementation can alleviate the clinical symptoms of perimenopause and reduce the risk of osteoporosis and cardiovascular disease, long-term application of exogenous estrogen also increases the incidence of breast cancer and endometrial cancer. The widespread application of hormone replacement therapy has been limited. Phytoestrogens are a series of ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/74A61P5/30A61P15/00
Inventor 崔明宇陈婷婷李艳凤吴伦马英丽
Owner HEILONGJIANG UNIV OF CHINESE MEDICINE
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