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Method for rapidly detecting diphacinone sodium salt, and detection kit thereof

An anti-mouse sodium salt, fast technology, applied in the field of analysis of food or health products, can solve the problems of false negative, perishable, difficult to observe the change of color, etc., and achieve the effect of high detection ability and high sensitivity

Inactive Publication Date: 2015-07-15
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The treatment method of the test sample is not given. Although the above method of preparing the test paper is simple, the concentration of the reagent used to prepare the test paper is high, which makes the prepared test paper yellow, and must be kept away from light and sealed, otherwise it is easy to deteriorate; It is difficult to observe the color change when the low concentration of diphacin sodium salt is positive, and it is easy to cause false negatives

Method used

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  • Method for rapidly detecting diphacinone sodium salt, and detection kit thereof
  • Method for rapidly detecting diphacinone sodium salt, and detection kit thereof
  • Method for rapidly detecting diphacinone sodium salt, and detection kit thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0055] Example 1. Preparation of Diratus Sodium Salt Rapid Detection Test Paper

[0056] Put the filter paper in a citric acid solution with a concentration of 0.1%, soak for 30 seconds, take it out, and dry at 80°C for 20 minutes, then place it in a 0.2% ferric ammonium sulfate solution, soak for 30 seconds, take it out, and dry at 80°C Dry for 20 minutes to obtain a test paper for the sodium salt of dirat, which can be referred to as test paper S1 in the present invention.

[0057] Take the filter paper and place it in a 0.5% glacial acetic acid solution, soak for 1 minute, take it out, and dry at 60°C for 30 minutes, then place it in a 0.2% ferric ammonium sulfate solution, soak for 2 minutes, take it out, and dry at 80°C Dry for 20 minutes to obtain a test paper for the sodium salt of Dirat, which can be referred to as test paper S2 in the present invention.

[0058] Take the filter paper and put it in 0.8% oxalic acid solution, soak for 30 seconds, take it out, dry at 50℃ for 4...

Embodiment 2

[0065] Example 2. General operating conditions for detecting whether the sample contains dirat sodium salt

[0066] 1. Sampling amount: 2~3g for solid and semi-solid samples; 2~3ml for liquid samples.

[0067] 2. Preparation and color development of the solution to be tested

[0068] (1) The colorless liquid sample can be directly immersed in the test paper of Diratus sodium salt in the solution to be tested for 3 seconds and taken out.

[0069] (2) For colored samples, you can take 2~3g or 2~3ml sample in the color developing tube, add 2~5ml ethyl acetate, shake for 1 minute, and take the supernatant as the test solution after standing. Immerse the test paper in the test solution for 3 seconds and take it out.

[0070] (3) For solid and semi-solid samples, you can take 2~3g sample in a color developing tube, add 2~5ml absolute ethanol or ethyl acetate, shake for 2 minutes, and take the supernatant as the test solution after standing still. The test paper is immersed in the test solut...

Embodiment 3

[0073] Example 3. Test for detecting whether the food and health products contain dirat sodium salt

[0074] The method of Example 2 was used to detect 10 batches of commercially available samples, and then the samples were tested according to the method of "High Performance Liquid Chromatography for the Detection of Di Rat Sodium in Food Poisoning Samples" published by Li Zhiling of Zhengzhou Center for Disease Control and Prevention.

[0075] The results are as follows:

[0076]

[0077]

[0078] The above-mentioned test results and high-performance liquid phase verification results show that the detection method of the present invention has no false positive report, and at the same time, no samples containing dirat sodium salt have been missed, and the results are accurate and reliable.

[0079] According to the method of the present invention, the 8 samples that were negative in the detection of the above-mentioned Dirat sodium salt were taken, respectively mixed with 5 mg of Dirat...

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Abstract

The invention relates to the technical field of food or health product analysis, and especially relates to a method for rapidly detecting diphacinone sodium salt, and a detection kit thereof. The method comprises the following steps: 1, taking a proper amount of a sample, mixing the sample with an extraction solvent, shaking, standing, and taking the above obtained supernatant; 2, taking diphacinone sodium salt test paper respectively dipped in an aqueous organic acid solution and an aqueous iron ammonium sulfate solution, immersing the diphacinone sodium salt test paper in the supernatant obtained in step 1, and taking out the test paper; or sucking or applying the supernatant obtained in the step 1 to the diphacinone sodium salt test paper; and 3, observing the color change of the test paper, determining that whether the detected sample contains diphacinone sodium salt or not, determining the detected sample possibly contains the diphacinone sodium salt if the test paper is light orange or orange red in color, and determining that the detected sample does not contain the diphacinone sodium salt if not. The method has the characteristics of small reagent use amount, rapidness, simplicity, safety and low pollution.

Description

Technical field [0001] The invention relates to the technical field of analysis of foods or health products, in particular to a method for rapid detection of dirat sodium salt based on chemical reaction and a detection kit thereof. Background technique [0002] Dimouse sodium salt is a new type of anticoagulant rodenticide commonly used at present. Its chemical name is 2-diphenylacetyl-1,3-indenedione. Its lethal dose is 0.5~2.5g. It has slow poisoning effect and inhibits Vitamin K 1 It can hinder the synthesis of prothrombin in the blood, damage the capillaries, increase the permeability and fragility of the tube wall, and easily rupture, causing massive internal organs and subcutaneous bleeding and death. The commonly used dosage form is 1% Dirat sodium salt powder. The clinical symptoms of early patients include nausea, vomiting, loss of appetite, lack of energy, joint swelling and pain, and low-grade fever; late onset is manifested as multiple bleeding throughout the body. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/78
Inventor 陈钦保
Owner ZHEJIANG UNIV
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