A kind of assay method of virus titer of chicken embryo virus liquid

Abstract

The invention discloses a chicken embryo virus liquid virus titer measurement method. The method includes the following steps of chicken embryo inoculation position determination, sample dilution, inoculation, hole sealing, chicken embryo post-incubation, cold egg acquisition of chicken embryos, chicken embryo erythrocyte agglutination titer measurement and EID50 calculation. By the adoption of the method, the chicken embryo inoculation position is changed, the amount of virus liquid injected in an allantoic cavity is more accurate, measurement result errors are reduced, in the hole sealing process, quick-setting glue is used for replacing wax adopted for hole sealing traditionally, the hole sealing speed is high, hole sealing operation is safe, in the cold egg acquisition process, a method of gradual transition of different temperatures is selected, the situation that yolks are broken due to chicken embryo struggles caused by the large temperature difference is avoided, and therefore the accuracy of a virus titer measurement result is improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a method for measuring the virus titer of chicken embryo virus liquid. Background technique [0002] There are following defects in the assay method of existing chicken embryo virus liquid virus titer: [0003] 1. The inoculation site of the chicken embryo should be about 3mm above the junction of the chicken embryo and the umbilical cord side and the air chamber. Since each person who draws the inoculation site and the inoculation personnel are different, the doubling distance drawn by each egg painter when drawing eggs is different. Although the distance between the side of the air chamber is different, the depth of the inoculation personnel's needle into the chicken embryo is different, which may easily cause the virus liquid to not completely enter the allantoic cavity of the chicken embryo during inoculation, and part of it is connected to the air chamber, resulting ...

AI Technical Summary

Problems solved by technology

[0003] 1. The inoculation site of the chicken embryo should be about 3mm above the junction of the chicken embryo and the umbilical cord side and the air chamber. Since each person who draws the inoculation site and the inoculation personnel are different, the doubling distance drawn by each egg painter when drawing eggs is different. Although the distance between the side of the air chamber is different, the depth of the inoculation personnel's needle into the chicken embryo is different, which may easily cause the virus liquid to not completely enter the allantoic cavity of the chicken embryo during inoculation, and part of it is connected to the air chamber, resulting in inconsistencies in the measurement results. big error

[0004] 2. The inoculated chicken embryos are sealed with wax. The wax is heated with a sprinkler lamp, and the temperature is high. When sealing on the chicken embryos, the high temperature will cause damage or death to the chicken embryos. At the same time, the wax sealing speed is relatively slow. low efficiency

[0005] 3. Chicken embryo harvesting and cold eggs, take out the mature chicken embryos from the greenhouse and put them directly into the 2-8 degree cold storage to cool the eggs, and suddenly turn the chicken embryos from the environment of 37 degrees to the environment of 2-8 degrees In the environment, the stress on the chicken embryo is relatively large, which may easily cause the chicken embryo to struggle hard and cause the yolk of the chicken embryo to rupture. Since the coagulation value is measured by taking the allantoic fluid of the chicken embryo, the allantoic fluid contains Egg yolk will affect the determination of hemagglutination value and the determination result of virus titer

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