Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Integrin receptor alpha-v-beta-3 related 5-peptide

一种整合素、肿瘤的技术，应用在体内试验用的配制品、肽、药物组合等方向，能够解决化药缺少肿瘤细胞特异性、器官毒性等问题

Active Publication Date: 2015-07-15
CHINA PHARM UNIV
View PDF1 Cites 5 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] At present, cancer has become one of the main causes of human death, and chemotherapy is still the main method for treating cancer. However, due to the lack of specificity of traditional chemical drugs for tumor cells, it has caused some organ toxicity to patients.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Integrin receptor alpha-v-beta-3 related 5-peptide
  • Integrin receptor alpha-v-beta-3 related 5-peptide
  • Integrin receptor alpha-v-beta-3 related 5-peptide

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] 5-peptide synthesis

[0023] 1: Coupling

[0024] Swell Fmoc-Met-Rink amide MBHA resin (Fmoc-Met-Rink amide-rink amino resin) 0.33 / 2mmol6.06g in dimethylformamide, 10ml / g resin, then use 20% six Hydropyridine / dimethylformamide solution removes the Fmoc protecting group (hereinafter referred to as decapping), washes the resin after decapping with dimethylformamide, adds raw material Fmoc-Asn(tBU)-OH 2.3g, condensing agent Use HBTU 1.44g, the reaction time is 30 minutes, and the Kaiser method is used for detection. If the test result is negative, it means that the coupling reaction is completed, and then the capping is carried out for 30 minutes. If the test result is still positive, it means that the coupling reaction is not completed. Or the reaction is not complete, it is necessary to prolong the coupling time or re-feed the coupling until the coupling reaction is completed. Repeat the above operations, sequentially coupling; Fmoc-D-Arg(Pbf)-OH; Fmoc-Trp-OH; Fmoc-Arg...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention belongs to the technical field of biomedicine production or the fields of protein polypeptide drugs and biomedical engineering, and in particular relates to a polypeptide which has high affinity with an integrin receptor and an application of the polypeptide like cancer diagnosis and treatment. The polypeptide is composed of five amino acids, and the sequence is arginine-tryptophan-arginine-asparagine-methionine (Arg-Trp-Arg-Asn-Met). On the basis of an in-vitro flow cytometry affinity determination experiment, the polypeptide disclosed by the invention has quite strong targeting on high-expression cells of the integrin receptor, and through an in-vitro laser confocal cell uptake experiment, the polypeptide can be targeted to high-expression tumor cells of the integrin receptor alpha-v-beta-3 and can basically avoid targeting on low-expression tumor cells and normal cells of the integrin receptor alpha-v-beta-3. An in-vitro cytotoxicity result shows that the 5-peptide disclosed by the invention is basically free from toxicity on cells, and the 5-peptide has a good application prospect in the diagnosis and the treatment of cancers.

Description

technical field [0001] The invention belongs to the field of bioengineering pharmaceutical technology, protein polypeptide medicine and biomedical engineering, and specifically relates to a polypeptide with high affinity for integrin receptor αvβ3 and its application, for example, in cancer diagnosis and treatment. Background technique [0002] At present, cancer has become one of the main causes of human death, and chemotherapy is still the main method to treat cancer. However, due to the lack of specificity of traditional chemical drugs against tumor cells, it has caused some organ toxicity to patients. [0003] Integrins are widely expressed in cells in the body, and most cells can express more than one integrin on the surface. These integrins not only participate in the growth, differentiation and apoptosis of some cells, but H plays a role It also plays a very important role in the pathological process. The integrin family includes 18 α subtypes and 8 β subtypes, and t...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12Q1/04A61K47/48A61P35/00
CPCA61K49/0041A61K49/0056A61P35/00C07K7/06G01N33/57492C07K14/70557G01N33/574
Inventor 顾月清张聪颖赵梦路王茜田彩平刘雨溪
Owner CHINA PHARM UNIV
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com