Pseudolarix plant regeneration method using pseudolarix leaves as explant

Abstract

The invention discloses a pseudolarix plant regeneration method using pseudolarix leaves as explants. The method comprises the following steps: producing callus through the induction of pseudolarix, performing the steps of bud differentiation culture, rooting culture, seedling hardening and transplanting on the callus in sequence, so as to finally obtain pseudolarix tissue culture seedlings with the high growth speed and the excellent property and without any pest and disease damage. A new path is provided for rapidly propagating the pseudolarix plant in large scale, and the problem that current pseudolarix medicinal material resources are shortage is effectively solved.

Description

technical field [0001] The invention relates to a method for regeneration of pine plants, in particular to a method for regeneration of pine plants using pine leaves as explants. Background technique [0002] The golden pine is an ancient and relict species of Pinaceae endemic to my country. According to the new threatened species classification system standards of the World Conservation Union (IUCN) and the standards of the Chinese Plant Red Book, the golden pine is designated as a endangered species and listed as a national second-class protected plant. [0003] The golden pine is not only an important garden plant, but also an important medicinal plant with broad application prospects. Studies have found that the most important medicinal ingredient of pine pine is the diterpenoid compound hibiscus acid extracted from its bark and root bark. It is the anti-tumor effect that makes the medicinal value of jinsong more prominent. Modern pharmacological research has found th...

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Problems solved by technology

[0005] At present, the research on the pine plant mainly focuses on the extraction of the effective components of the pine and the screening of the endophytic fungi, and in the research report of plant biotechnology, there is "a kind of leaf primordium as the The research reports on the culture method of pine callus from explants” (patent number: ZL 201210104866.8) and “a method for suspension culture of pine cells” (patent number: ZL 201210472291.5); however, the above two patents use money The buds and leaf primordia of pine plants are used as explants, and the callus induction and proliferation culture have been completed through solid and liquid culture methods. At present, it has not involved the use of pine leaves as explants. After the callus, further complete the differentiation of adventitious buds and the rooting culture of rootless seedlings, and finally successfully cultivated the report of tissue culture seedlings of pine plants