Rosa roxburghii ex-vivo twig culturing method and application thereof in stony desertification control
A technology of rocky desertification and branches, applied in the fields of application, botany equipment and methods, horticulture, etc., can solve problems such as poor water conditions, inapplicability, and poor soil, and achieve increased vegetation coverage, low cost, and no soil pollution effect
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Embodiment 1
[0037] A method for growing seedlings of roxburghii in vitro, comprising the steps of:
[0038] Step (1), the selection and processing of mother branch: select the robust biennial and perennial thorn pear branch as the mother branch of seedling cultivation, cut it into 15 cm long stem segments, each with 1 bud point;
[0039] Step (2), raising seedlings: wrap the lower end of the stem segment obtained in step (1) with a wet absorbent material soaked in 50mg / L indole acetic acid solution, then place it in the culture cup, and place the culture plate Or put the culture cup into a light-transmitting container, place it at room temperature, add water every other day, and keep the gauze wrapped at the lower end of the branch moist. After 15 days, more than 90% of the stems will take root and germinate to become a complete seedling; The material is cotton, filter paper or cotton gauze;
[0040] Step (3), transplanting and hardening: transplant the seedlings obtained in step (2) int...
Embodiment 2
[0048] A method for growing seedlings of roxburghii in vitro, comprising the steps of:
[0049] Step (1), the selection and processing of female branch: select the robust biennial and perennial prickly pear branch as the female branch of seedling cultivation, cut it into 20 cm long stem segments, each with 3 bud points;
[0050] Step (2), raising seedlings: wrap the lower end of the stem segment obtained in step (1) with a wet absorbent material soaked with 50mg / L of indole acetic acid solution, then place it in a culture tray, and place the culture tray Or put the culture cup into a light-transmitting container, place it at room temperature, add water every 2 days, and keep the gauze wrapped at the lower end of the branch moist. After 15 days, more than 90% of the stems will take root and germinate to become a complete seedling; The material is cotton, filter paper or cotton gauze;
[0051] Step (3), transplanting and hardening: transplant the seedlings obtained in step (2) ...
Embodiment 3
[0059] A method for growing seedlings of roxburghii in vitro, comprising the steps of:
[0060] Step (1), the selection and processing of mother branch: select the robust biennial and perennial thorn pear branch as the mother branch of seedling cultivation, cut it into 18 cm long stem segments, each with 2 bud points;
[0061] Step (2), raising seedlings: wrap the lower end of the stem segment obtained in step (1) with a wet absorbent material soaked with 50mg / L of indole acetic acid solution, then place it in a culture tray, and place the culture tray Or put the culture cup into a light-transmitting container, place it at room temperature, add water every 1.3 days, and keep the gauze wrapped at the lower end of the branch moist. After 15 days, more than 90% of the stems will take root and germinate to become a complete seedling; The material is cotton, filter paper or cotton gauze;
[0062] Step (3), transplanting and hardening: transplant the seedlings obtained in step (2) ...
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Abstract
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Application Information
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