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A method for producing ethanol from lignocellulose by natural pH fermentation

A technology of lignocellulose and ethanol production, which is applied in the field of microbial fermentation, can solve the problems of complex operation, high content of miscellaneous bacteria, and affecting fermentation yield, and achieve good fermentation performance, high ethanol output, and avoid complex operations.

Active Publication Date: 2020-05-12
COFCO NUTRITION & HEALTH RES INST +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

But generally there are following problems: 1) in order to make the fermentation strain in the optimum pH environment, have stronger activity, to improve the output of ethanol, have to add appropriate alkali to real-time adjustment to the pH of fermented liquid in the fermentation process, this not only It makes the operation in the fermentation process complicated, and it is very easy to affect the fermentation yield due to the error of regulation, and in the environment of adding alkali, it is beneficial to the growth of miscellaneous bacteria such as lactic acid bacteria, and it is easy to cause the content of miscellaneous bacteria in the fermentation system to be high

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  • A method for producing ethanol from lignocellulose by natural pH fermentation

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Embodiment 1

[0034] The method for producing ethanol from lignocellulose at natural pH of the present embodiment comprises the following steps:

[0035] 1) Take the Ho-Purdue yeast 424A (LNH-ST) strain for seed cultivation and expansion cultivation; the cultivation process is as follows:

[0036] Seed culture: 10g / L yeast powder, 20g / L peptone, and 20g / L glucose were mixed and sterilized to prepare a seed liquid medium, and the Ho-Purdue yeast 424A (LNH-ST) strain was inoculated into the The culture medium mentioned above was cultivated for 20 hours until the strain concentration reached (0.2-0.3)×10 9 / ml.

[0037] Expansion cultivation: take the bacteria obtained in the seed cultivation, inoculate 5% of the inoculum into the shake flask containing the expansion culture medium, the expansion temperature is 30°C, the rotation speed is 200rpm, the cultivation time is 16h, and the first-level bacteria are 5 % inoculated into the second-stage expansion culture medium, adjust the pH value to...

Embodiment 2

[0043] The method for producing ethanol from lignocellulose at natural pH of the present embodiment comprises the following steps:

[0044] 1) Take the Ho-Purdue yeast 424A (LNH-ST) strain for seed cultivation and expansion cultivation; the cultivation process is as follows:

[0045] Seed culture: 10g / L yeast powder, 20g / L peptone, and 20g / L glucose were mixed and sterilized to prepare a seed liquid medium, and the Ho-Purdue yeast 424A (LNH-ST) strain was inoculated into the culture medium at 30°C and pH 6.0 until the strain concentration reaches (0.1-0.2)×10 9 / ml.

[0046] Expansion cultivation: take the bacteria obtained in the seed cultivation, inoculate 20% of the inoculum into the shake flask containing the expansion culture medium, adjust the pH value to 4, expand the cultivation temperature at 35°C, rotate at 100rpm, and cultivate for 14 hours. Expand the culture to (0.2-0.3) × 10 9 / ml.

[0047] The medium for the expanded culture is sweet sorghum stalk juice cont...

Embodiment 3

[0052] The method for producing ethanol from lignocellulose at natural pH of the present embodiment comprises the following steps:

[0053] 1) Take the Ho-Purdue yeast 424A (LNH-ST) strain for seed cultivation and expansion cultivation; the cultivation process is as follows:

[0054] Seed culture: 10g / L yeast powder, 20g / L peptone, and 20g / L glucose were mixed and sterilized to prepare a seed liquid medium, and the Ho-Purdue yeast 424A (LNH-ST) strain was inoculated into the The culture medium mentioned above was cultivated until the strain concentration reached (0.4-0.5)×10 9 / ml.

[0055] Expansion cultivation: take the bacteria obtained in the seed cultivation, inoculate 10% of the inoculum into the shake flask containing the expansion culture medium, adjust the pH to 6, expand the cultivation temperature at 28°C, rotate at 150rpm, cultivate for 14 hours, expand Cultivate to (0.2-0.3)×10 9 / ml.

[0056] The culture medium of described expansion is the molasses culture m...

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Abstract

The invention discloses a method for generatinbg ethyl alcohol through natural pH fermentation of lignocellulose. Specific induction cultivation is carried out on specific strains by virtue of a selected amplification culture medium which take contained C5 and C6 as carbon sources, and the strains have good fermentation activity, natural pH fermentation can be realized, and meanwhile the fermentation performance of the strains is good. Meanwhile, meta-acid of a fermentable system can effectively restrain growth of infectious microbes like lactic acid bacteria, and the concentration of lactic acid, acetic acid and the like generated by the infectious microbe in the fermentation liquid is obviously reduced.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for producing ethanol from lignocellulose with natural pH. Background technique [0002] The depletion of many non-renewable fossil energy sources such as oil has drawn more and more attention to renewable energy, especially biofuels, and has brought huge business opportunities and social significance. [0003] Ethanol is a clean and renewable liquid fuel. Many countries have begun to use gasoline-gasoline alcohol added with a certain proportion of ethanol to replace the consumption of gasoline. This new type of fuel can not only alleviate the consumption rate of oil, but also reduce the pollution of automobile exhaust, and has great application and development potential. Since 2001, my country has started to promote the use of gasoline alcohol. At present, gasoline alcohol accounts for about 20% of the total gasoline fuel consumption, and it is growing...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10C12P19/14C12R1/74
CPCC12P7/10C12P19/14Y02E50/10
Inventor 李春玲魏拥辉杨宇平李杰范洪岩张宁吴杨宋思琦董敬波李凡林新沈乃东
Owner COFCO NUTRITION & HEALTH RES INST
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