Preparation method and application of removing agent for heavy metal cadmium in mussels

A technology of heavy metal and remover, applied in the application, food preparation, food science and other directions, to achieve the effect of simple process, enhanced immunity, and simple process operation

Inactive Publication Date: 2015-07-01
ZHEJIANG OCEAN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, there has been no report on the removal of heavy metal cadmium from mussels in rearing.

Method used

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  • Preparation method and application of removing agent for heavy metal cadmium in mussels
  • Preparation method and application of removing agent for heavy metal cadmium in mussels
  • Preparation method and application of removing agent for heavy metal cadmium in mussels

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A kind of preparation method of heavy metal cadmium removing agent in mussel, concrete steps are as follows:

[0023] 1. Take hairtail processing by-products, add phosphate buffer solution with a pH value of 7.0 and a concentration of 0.18mol / L. The amount of phosphate buffer solution added is 5 times the volume of hairtail processing by-products, and the tissues are crushed and ultrasonically treated at 37KHz for 4 minutes. Finally, add 8g / L of compound hydrolase, which is composed of flavor protease and papain in a mass ratio of 2:1, the hydrolysis temperature is controlled at 50°C, and the degree of protein hydrolysis is 28%;

[0024] 2. Raise the temperature to 90°C and keep it for 2.5 minutes, then quickly cool it in an ice bath, centrifuge at 4000r / min, and centrifuge for 15 minutes to obtain the supernatant, which is the hairtail processing by-product protein hydrolyzate;

[0025] 3. Add sodium selenite to hairtail processing by-product protein hydrolyzate (the a...

Embodiment 2

[0032] A kind of preparation method of heavy metal cadmium removing agent in mussel, concrete steps are as follows:

[0033] 1. Take hairtail processing by-products, add phosphate buffer solution with a pH value of 6.8 and a concentration of 0.05mol / L, the amount of phosphate buffer added is 6 times the volume of hairtail processing by-products, break the tissue, and 30KHz ultrasonic treatment for 5 minutes Finally, add 10g / L of compound hydrolase, which is composed of flavor protease and papain in a mass ratio of 1:1, the hydrolysis temperature is controlled at 40°C, and the degree of protein hydrolysis is 24%;

[0034] 2. Raise the temperature to 85°C, keep it for 3 minutes, cool it in an ice bath, centrifuge at 4500r / min, and centrifuge for 10 minutes to obtain the supernatant, which is the protein hydrolyzate by-product of hairtail processing;

[0035] 3. Add sodium selenite to hairtail processing by-product protein hydrolyzate (the addition amount is controlled so that th...

Embodiment 3

[0042] A kind of preparation method of heavy metal cadmium removing agent in mussel, concrete steps are as follows:

[0043]1. Take hairtail processing by-products, add phosphate buffer solution with a pH value of 7.4 and a concentration of 0.20 mol / L, the amount of phosphate buffer added is 3 times the volume of hairtail processing by-products, crush the tissue, and ultrasonically treat at 45KHz for 2 minutes Finally, 6g / L of compound hydrolase was added. The compound hydrolase was mixed with flavor protease and papain at a mass ratio of 3:1. The hydrolysis temperature was controlled at 60°C, and the degree of protein hydrolysis was 32%;

[0044] 2. Raise the temperature to 95°C, keep it for 2 minutes, then quickly cool it in an ice bath, centrifuge at 3000r / min, and centrifuge for 20 minutes to obtain the supernatant, which is the hairtail processing by-product protein hydrolyzate;

[0045] 3. Add sodium selenite to hairtail processing by-product protein hydrolyzate (the add...

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Abstract

The invention provides a preparation method of a removing agent for heavy metal cadmium in mussels. The preparation method comprises the following steps: adding the taken processing byproducts of hairtails into a phosphoric acid buffering solution; carrying out tissue crushing and ultrasonic treatment; then adding compound hydrolase to hydrolyze; controlling the temperature to 40-60 DEG C and the protein hydrolysis degree to be 24%-32%; after hydrolyzing, heating to 85-95 DEG C and keeping for 2.0-3.0 minutes; rapidly cooling in a water bath, and carrying out centrifugal separation to obtain liquid supernatant, namely protein hydrolysis liquid of the processing byproducts of the hairtails; adding sodium selenite, wherein the adding amount is that the Se content in a mixing system is 50-90 micrograms/liter; adjusting the pH of a chelating reaction system to 4.0-6.0 and reacting at 20-35 DEG C for 35-50 minutes; after the reaction is finished, carrying out centrifugal separation; and taking out liquid supernatant, carrying out vacuum concentration and drying to obtain a protein hydrolysis-peptide-Se element compound. According to the preparation method, the process is simple and the raw material cost is low; the prepared removing agent is natural and environmentally friendly, is safe and reliable to use, and does not cause secondary pollution; and the heavy metal cadmium enriched in the mussels can be effectively removed and the eating safety of people is ensured.

Description

technical field [0001] The invention relates to a preparation method of a heavy metal cadmium removing agent in mussels. Background technique [0002] Bivalve mussels are a kind of marine molluscs, including blue mussels, emerald mussels, thick-shell mussels, etc. Its nutritional value is very high, among which the protein content is as high as 59%, contains 8 kinds of essential amino acids, and the fat content 7%, and most of them are unsaturated fatty acids. In addition, it is also rich in calcium, phosphorus, iron, zinc and vitamin B, niacin and so on. Because the nutrients contained in mussels are very rich, its nutritional value is higher than that of ordinary shellfish, fish, shrimp, meat, etc., and it has a positive effect on promoting metabolism and ensuring the nutrient supply for brain and body activities, so some people call it mussels. Bei means "egg in the sea". [0003] However, in recent years, the pollution of heavy metals to organisms, especially bottom-d...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/015A23L1/33A23L5/20A23L17/40
Inventor 张宾房传栋吴海潇邹俊杰邓尚贵
Owner ZHEJIANG OCEAN UNIV
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