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Application of hyperbranched polyglycerol modified nanosphere to immunochromatography

A technology of glycidyl ether and hyperbranched polymer, which is applied in the field of biomedical diagnosis, can solve the problems of limited application, weak binding ability, and low coupling efficiency, and achieve the effects of reducing non-specific binding, broadening the detection range, and improving detection quality

Inactive Publication Date: 2015-06-24
GETEIN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, since the linear PEG polymer has functional groups only in the polymer end group in its structure, there are only one or two active sites available for coupling in the entire polymer structure, the binding ability is weak, and the coupling efficiency is not high. This limits its wider application

Method used

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  • Application of hyperbranched polyglycerol modified nanosphere to immunochromatography
  • Application of hyperbranched polyglycerol modified nanosphere to immunochromatography
  • Application of hyperbranched polyglycerol modified nanosphere to immunochromatography

Examples

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Effect test

Embodiment 1

[0042] Example 1: Preparation method of hyperbranched polyglycidyl ether modified nanosphere dry immunochromatography test strip

[0043] 1. Preparation of hyperbranched polyglycidyl ether modified nanospheres:

[0044] 1) Preparation of hyperbranched polyglycidyl ether: In a three-necked flask equipped with mechanical stirring and a micro constant flow pump, add 0.24g trimethylol propane (TMP) as the initiator of the reaction, using 3.7M potassium methylate (CH3OK) deprotonates the hydroxyl part of TMP, the deprotonated part accounts for 10% of the total part, and the excess methanol is removed by heating and evaporating; then slowly add 50ml of glycidol into the system to initiate polymerization, pay attention to the dropping speed, too fast may Cause implosion, reaction temperature 95°C, reaction time 12h;

[0045] After the reaction, the product was dissolved in methanol and passed through a strong acid cation exchange resin (AMBERJET TM 1500H Resin) polymer is precipit...

Embodiment 2

[0056] The method for making test strips as a control group in embodiment 2 is the same as the above method, except that commercialized polystyrene microspheres (the same as the experimental control, the manufacturer's JSR diagnostic reagent, article number MS 160 / Carboxyl) do not hyperbranched grooming. Example 2: Evaluation of detection indicators of hyperbranched polyglycidyl ether modified nanospheres dry immunochromatography test strips

[0057] The performance analysis of the reagents was carried out on the cardiac troponin I (cTnI) project using hyperbranched glycidyl ether modified nano-microsphere immunochromatographic test strips. The experiment on the I(cTnI) project was used as a control group. During the experiment, 50ul of the analyte was added from the sample pad, and after 15min, its fluorescence value was measured by Getein1100 fluorescence immunoassay analyzer (Nanjing Jidan Biotechnology Co., Ltd.), and then Calculate its related performance indicators.

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Abstract

The invention discloses an application of a hyperbranched polyglycerol modified nanosphere to immunochromatography. In the application of the hyperbranched polyglycerol modified nanosphere to immunochromatography disclosed by the invention, a hydrophilic hyperbranched modified nanosphere material rich in functional groups is prepared by means of the hydrophilic performance of hyperbranched polyglycerol, and an immunochromatographic test strip of hyperbranched polyglycerol modified nanosphere is prepared in combination with dry immunochromatography. In practical application, when the test strip established by the system disclosed by the invention is applied to disease detection, compared with a common microsphere test strip, the test strip is stronger in sensitivity, wide in detection range and excellent in performance, and correlation, repeatability and stability satisfy the production requirements.

Description

technical field [0001] The invention relates to the field of biomedical diagnosis, in particular to the application of hyperbranched polyglycidyl ether modified nanometer microspheres in immunochromatography. Background technique [0002] Immunochromatography technology uses the capillary action of the liquid to allow the analyte to undergo a specific immune reaction at the detection line through chromatography, and obtain the judgment result through naked eye or corresponding instrument detection. It combines affinity technology, labeling technology, imprinting technology and chromatography technology and other technical methods. It is an important method for preparing in vitro diagnostic reagents, and has the characteristics of low price and rapid detection. Another advantage of immunochromatography is that it requires almost no complicated equipment, has convenient user operability, and is in line with the strategy of point-of-care testing (POCT). It has become a strong ...

Claims

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Application Information

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IPC IPC(8): G01N33/531G01N33/533
CPCG01N33/558G01N33/54346
Inventor 金晶罗雅赛黄力朱宗哲苏恩本
Owner GETEIN BIOTECH
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