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Identification method of aliphatic chain isomer alpha-oxoglutarate and 1, 3-acetone dicarboxylic acid

A technology of isomers and acetone dicarboxylic acid, which is applied in the field of discrimination and identification, can solve the problems of rare identification methods and difficulty in distinguishing, and achieve the effects of easy operation and observation, high accuracy and simple equipment

Inactive Publication Date: 2015-06-24
ANHUI UNIVERSITY
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Problems solved by technology

[0003] Since α-ketoglutaric acid and 1,3-acetone dicarboxylic acid have the same molecular formula and similar structure, some physical and chemical properties are also similar, and the appearance of the two is also very similar, making it difficult to distinguish between the two
At present, the content of α-ketoglutaric acid is usually determined by instrumental analysis methods such as reversed-phase high-performance liquid chromatography (RP-HPLC) and reversed-phase ion-pair chromatography (IPC), or by paper chromatography (PC). Analysis and identification; but there are few reports on the determination or identification method of 1,3-acetone dicarboxylic acid and the identification method between α-ketoglutaric acid and 1,3-acetone dicarboxylic acid

Method used

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  • Identification method of aliphatic chain isomer alpha-oxoglutarate and 1, 3-acetone dicarboxylic acid
  • Identification method of aliphatic chain isomer alpha-oxoglutarate and 1, 3-acetone dicarboxylic acid
  • Identification method of aliphatic chain isomer alpha-oxoglutarate and 1, 3-acetone dicarboxylic acid

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0052] This example verifies the feasibility of the method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetonedicarboxylic acid according to the following steps:

[0053] (1) Prepare the solution

[0054] First prepare the sulfuric acid of 0.025mol / L with 98% vitriol oil and distilled water as stock solution, then prepare respectively the potassium iodate solution of 0.14mol / L, the [NiL] of 0.0173mol / L with the sulfuric acid solution of 0.025mol / L ( ClO 4 ) 2 solution, 2mol / L malonic acid solution, 4mol / L hydrogen peroxide solution; add 14.5mL 0.025mol / L sulfuric acid solution, 6.5mL 0.14mol / L potassium iodate solution, 1.5mL 0.0173 mol / L[NiL](ClO 4 ) 2 solution, 3.5mL 2mol / L malonic acid solution and 14mL 4mol / L hydrogen peroxide solution to ensure that "H 2 SO 4 -KIO 3 -[NiL](ClO 4 ) 2 -MA-H 2 o 2 "The concentration of each component in the nonlinear chemical oscillation system is sulfuric acid 0.025mol / L, potassium iodate 0.02275mol / L, [Ni...

Embodiment 2

[0064] This example verifies the feasibility of the method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetonedicarboxylic acid according to the following steps:

[0065] (1) Prepare the solution

[0066] First prepare 0.025mol / L sulfuric acid with 98% concentrated sulfuric acid as a stock solution, then prepare 0.14mol / L potassium iodate solution and 0.0173mol / L [NiL](ClO 4 ) 2 solution, 2mol / L malonic acid solution, 4mol / L hydrogen peroxide solution; add 13.5mL 0.025mol / L sulfuric acid solution, 6.5mL 0.14mol / L potassium iodate solution, 2mL 0.0173mol / L[NiL](ClO 4 ) 2 solution, 3.5mL 2mol / L malonic acid solution and 14.5mL 4mol / L hydrogen peroxide solution to ensure that "H 2 SO 4 -KIO 3 -[NiL](ClO 4 ) 2 -MA-H 2 o 2 "The concentration of each component in the nonlinear chemical oscillation system is sulfuric acid 0.025mol / L, potassium iodate 0.02275mol / L, [NiL](ClO 4 ) 2 8.65×10 -4 mol / L, malonic acid 0.175mol / L, hydrogen peroxide 1.45mo...

Embodiment 3

[0074] This example verifies the feasibility of the method for identifying aliphatic chain isomers α-ketoglutaric acid and 1,3-acetonedicarboxylic acid according to the following steps:

[0075] (1) Prepare the solution

[0076] First prepare 0.025mol / L sulfuric acid with 98% concentrated sulfuric acid as a stock solution, then prepare 0.14mol / L potassium iodate solution and 0.0173mol / L [NiL](ClO 4 ) 2 solution, 2mol / L malonic acid solution, 4mol / L hydrogen peroxide solution; add 15.5mL 0.025mol / L sulfuric acid solution, 6mL 0.14mol / L potassium iodate solution, 1.5mL 0.0173mol / L[NiL](ClO 4 ) 2 solution, 3mL 2mol / L malonic acid solution and 14mL 4mol / L hydrogen peroxide solution to ensure that "H 2 SO 4 -KIO 3 -[NiL](ClO 4 ) 2 -MA-H 2 o 2 "The concentration of each component in the nonlinear chemical oscillation system is sulfuric acid 0.025mol / L, potassium iodate 0.021mol / L, [NiL](ClO 4 ) 2 6.4875×10 -4 mol / L, malonic acid 0.15mol / L, hydrogen peroxide 1.4mol / L; a...

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Abstract

The invention discloses an identification method of aliphatic chain isomer alpha-oxoglutarate and 1,3-acetone dicarboxylic acid. The identification method is characterized by comprising the following steps that with an H<2>SO<4>-KIO<3>-[NiL](ClO<4>)<2>-MA-H<2>O<2> nonlinear chemical oscillation system as an identification solution, differential identification of the aliphatic chain isomer is realized according to an oscillation response of the system generated by the aliphatic chain isomer; L in [NiL](ClO<4>)<2> is 5, 7, 7, 12, 14, 14-hexamethyl-1, 4, 8, 11-tetraaza tetradecyl-4, 11-diene. An oscillation map provided by the identification method is relatively high in intuition; moreover, the aliphatic chain isomer alpha-oxoglutarate and 1,3-acetone dicarboxylic acid can be conveniently and quickly identified, and the method can also be widely used in other isomers; in addition, identification equipment is simple, high in accuracy and easy to operate and observe.

Description

technical field [0001] The present invention relates to a kind of distinguishing and identifying method, specifically a kind of nickel complex [NiL](ClO 4 ) 2 A method for the identification of the aliphatic chain isomers α-ketoglutarate and 1,3-acetonedicarboxylic acid by a catalytic nonlinear chemical system. technical background [0002] α-Ketoglutaric acid and 1,3-acetone dicarboxylic acid have the same molecular formula, are aliphatic chain isomers, and play a pivotal role in their respective fields. As one of the intermediate products of the tricarboxylic acid cycle in the living body, α-ketoglutarate is not only widely used to determine the substrates of aminotransferases and dehydrogenases and supporting reagents for the determination of liver function, but also can be used in organic synthesis Intermediate, in addition, it is also an important additive in medicine, health products, and food; and 1,3-acetone dicarboxylic acid is one of the important pharmaceutical ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/26
Inventor 胡刚张宇胡娟娟胡彦旸刘婷婷胡林宋继梅
Owner ANHUI UNIVERSITY
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