Super-high-temperature-resistant and alkali-resistant beta-mannase gene, amino acid sequence and application of beta-mannase gene

Abstract

The invention discloses a super-high-temperature-resistant and alkali-resistant beta-mannase gene. A base sequence of the beta-mannase gene is SEQ ID NO.1. The invention further discloses a nucleotide sequence SEQ ID NO.2 of super-high-temperature-resistant and alkali-resistant beta-mannase, and further discloses application of the super-high-temperature-resistant and alkali-resistant beta-mannase gene in preparation of the super-high-temperature-resistant and alkali-resistant beta-mannase. The obtained expression enzyme can widely endure alkaline (pH7-10) and high-temperature (80-100 DEG C) environments; and the specific activity of the purified expression enzyme can be up to 10400U / mg. The beta-mannase has super-high-temperature-resistant and alkali-resistant characteristics, and is suitable for application in the chemical industries such as biomass degradation, foods, feeds, papermaking and spinning.

Description

technical field [0001] The invention belongs to the technical field of microbial enzyme engineering, and specifically relates to an ultra-high temperature and alkali-resistant β-mannanase gene. The invention also discloses an amino acid sequence of an ultra-high temperature and alkali-resistant β-mannanase. Also disclosed is the application of an ultrahigh temperature and alkali resistant β-mannanase gene. Background technique [0002] Mannan is the main component of hemicellulose in biomass, and β-mannanase plays an important role in the development and utilization of natural hemicellulose resources and the research and development of mannan-oligosaccharide drugs. β-mannanase (β-1,4-mannan mannohydrolase, EC 3.2.1.78), also known as β-D-mannanase or β-1,4-D-mannanase, is a class of Endohydrolase capable of hydrolyzing mannan oligosaccharides and mannan polysaccharides (including mannan, galactomannan, glucomannan, etc.) containing β-1,4-D-mannosidic bonds. It belongs to t...

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Problems solved by technology

The research on β-mannanase in my country has been started since the 1980s, and related enzymes have also been reported, but they belong to the thermophilic β-mannanase of the glycan hydrolase superfamily 26 (Glyco_hydro_26 superfamily) The temperature is 70-100°C and the pH range of alkali resistance is 7-10, which has not been reported in China.

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