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Method for improving zinc-enrichment of beer yeast through ultra-high pressure mutant

A technology of brewer's yeast and brewer's yeast, applied in the direction of microorganism-based methods, biochemical equipment and methods, mutant preparation, etc., can solve the problems of limited application, less research on zinc-rich yeast breeding, etc., and achieve obvious results , Improve the ability of zinc enrichment, and the effect of simple operation

Inactive Publication Date: 2015-06-24
马龙
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Problems solved by technology

Common mutagenesis methods such as UV light and chemical mutagens largely limit their application due to their radiation or toxicity
With the development of high-pressure theory and technology, the research of high-pressure biology has penetrated into many fields of biology. Ultra-high pressure mutagenesis technology has made new progress in industrial microbial breeding, but the use of ultra-high pressure technology to produce zinc-enriched yeast Less research on selection

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0010] Mutagenesis procedure:

[0011] Activation of the starting strain of brewer's yeast → ultra-high pressure mutagenesis of bacterial suspension → primary screening of zinc-containing selective medium → domestication of zinc resistance → obtaining high zinc-rich strains.

[0012] Pick two rings of beer yeast starting bacteria from the slant of the preservation test tube, put them in a 250mL Erlenmeyer flask filled with 50mL YPD liquid medium, and culture them at 28°C and 150rpm for 10h to reach the logarithmic growth phase. Take 10 mL of cultured brewer's yeast liquid, centrifuge at 4°C and 4000 rpm for 10 min, and wash with sterile saline three times to obtain a bacterial cell suspension. Shake gently in the shaker for 30 minutes before use to allow the bacteria to disperse evenly. Dilute the prepared bacterial suspension appropriately, and adjust the cell concentration to 10 with a hemocytometer. 6 pc / mL, spare.

[0013] Take 5 mL of brewer’s yeast suspension under as...

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Abstract

The invention discloses a method for improving zinc-enrichment of beer yeast through ultra-high pressure mutant, belonging to the technical field of microbial breeding. Beer yeast original strains are subjected to activation, ultra-high pressure mutant treatment of bacterium suspension cells is performed, and mutant strains are subjected to screening and acclimation by utilizing zinc-containing selective medium, so that zinc-enrichment of the beer yeast is improved. The high zinc-rich beer yeast mutant strains are obtained through ultra-high pressure mutant, and the method is safer, simpler in operation and more obvious in effect compared to ultraviolet and chemical mutagen mutant. The tolerance concentration to zinc of the beer yeast mutant strains reaches 3.2 mmol / L, and the zinc content reaches 2.75 mg / g, and is higher than the zinc content of beer yeast original strains by 6.55 times.

Description

technical field [0001] The invention relates to a method for improving the zinc-rich ability of brewer's yeast through ultra-high pressure mutagenesis, which belongs to the technical field of microbial breeding, and particularly refers to a method of using a zinc-containing selective medium to treat brewer's yeast cells with ultra-high pressure for mutagenesis. A method for screening and acclimating mutant strains and improving the zinc-enriching ability of brewer's yeast. Background technique [0002] Zinc, as one of the essential trace elements for the human body, has attracted more and more attention. Zinc is mainly absorbed and metabolized in the form of organic state in the human body, while inorganic zinc cannot be digested and absorbed by the human body, and has certain toxicity. The use of microorganisms to enrich the essential trace elements of humans and animals, and to manufacture low-cost and safe functional foods is also increasingly favored by people. Yeast h...

Claims

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Application Information

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IPC IPC(8): C12N15/01C12R1/865
Inventor 马龙许秀勤
Owner 马龙
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