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A strain of Serratia marcescens resistant to X. citrus

A technology for citrus canker, Serratia marcescens, in bacteria, microbe-based methods, chemicals for biological control, etc.

Inactive Publication Date: 2016-04-06
HUNAN AGRICULTURAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Rossetti (1977), Civerolo (1981), Schoulties (1987), Civerolo (1991) and Goto (1992) all expounded the views on the prevention and treatment of ulcer disease in their studies, but their studies were all restricted by specific regions. Restrictions may be outdated in today's era of deep communication

Method used

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  • A strain of Serratia marcescens resistant to X. citrus
  • A strain of Serratia marcescens resistant to X. citrus
  • A strain of Serratia marcescens resistant to X. citrus

Examples

Experimental program
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Effect test

Embodiment 1

[0029] Embodiment 1 Preparation of antagonistic bacterial strain T861 of the present invention

[0030] Collect soil samples from Longshan County, Xiangxi Autonomous Prefecture. Take 10g of each soil sample, add them to a triangular flask filled with 90ml sterile water (with glass beads), shake for 1 hour, filter to remove sediment, and use sterile water to obtain the bacterial suspension. Carry out serial dilution, successively dilute to 10 -2 、10 -3 、10 -4 、10 -5 、10 -6 . Bacteria were separated by mixing method, and fungi and actinomycetes were separated by smear method. Cultivate at 28°C for 48 hours, select colonies with different shapes, sizes, and colors, purify them, and store them in slant medium.

[0031] The citrus canker sore bacteria is mixed with the melted culture medium and poured into a sterilized petri dish, a hole is punched on the solidified gel plate, and the isolated strains are made into a certain concentration of bacterial suspension , use a pipe...

Embodiment 2

[0032] Example 2 Indoor inhibitory effect of bacterial strain T861 of the present invention on canker sore citrus

[0033] Mix the bacteria liquid made by X. citrus canker sore with the melted culture medium, pour it into a sterilized petri dish, make a hole on the solidified gel plate, and make the isolated T861 strains into suspensions of certain concentrations. solution, use a pipette gun to take 100ul of the bacterial solution and add it to the round hole of the corresponding plate, and set up 3 parallel holes. Place the marked Petri dish in a 30°C incubator, and observe the formation of the inhibition zone every 24 hours. The average value of the diameter of the inhibition zone formed by strain T861 is about 55mm, and the edge of the inhibition zone is slightly raised, see figure 2 .

Embodiment 3

[0034] Example 3 Bacterial strain T861 of the present invention is used for preventing and treating canker sores of citrus on living citrus

[0035] The bacterial strain T861 of the present invention was picked and inoculated into 25ml of liquid LB liquid medium, cultured at 37°C and 180r / min for 24h to obtain a fermentation broth. Select 3 citrus trees with similar growth conditions, select four fully expanded new leaves with similar growth and development levels on each tree, and puncture the upper epidermis of the leaves with a sterilized inoculation needle (20 holes per leaf). Spray X. citrus bacteria on the upper surface and lower surface of the blade by spraying method. After 1 day, spray the fermented liquid of the bacterial strain T861 of the present invention on 3 new leaves of citrus treated by X. citrus. , do not spray the fermented liquid of bacterial strain of the present invention but spray clear water on the control blade, observe the disease situation after spr...

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PUM

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Abstract

A strain of Serratia marcescens (Serratia marcescens) T861 resistant to canker sores of citrus was preserved in the China Center for Type Culture Collection on April 10, 2015, with the preservation number CCTCC? NO: M2015222. The fermented liquid of the bacterial strain T861 of the present invention has inhibitory effect on X. citrus bacteria, and the in vitro inhibition rate of the aseptic fermented liquid on X. citrus bacteria is more than 86.7% as measured by the acupuncture method.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a Serratia marcescens bacterial strain resistant to canker sores of citrus, in particular to a Serratia marcescens (Serratia marcescens) T861 strain, and at the same time relates to the effect of the strain on preventing and treating canker citrus application. Background technique [0002] Citrus canker is a quarantine disease caused by Xanthomonasaxonopodispv. Citri, which seriously affects the quality and yield of citrus. It has been reported in most parts of the world where citrus crops grow. Citrus canker is a bacterial disease, which has the characteristics of fast infection, wide transmission route, high degree of damage, and stubborn disease. Although countries around the world have formulated strict regulations and adopted various measures to block the introduction of canker disease, the adjustment of agricultural and forestry industry structure and the development of economic...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P1/00C12R1/43
CPCA01N63/00A01N63/10C12N1/205C12R2001/43
Inventor 唐前君罗葵戴良英周志成肖启明刘双清李魏李迅
Owner HUNAN AGRICULTURAL UNIV
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