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Feed Paenibacillus and its application

A bacillus and feed technology, applied in the direction of application, bacteria, fungicides, etc., can solve the problems of difficulty in the control of chemical agents, large genetic variation of tobacco powdery mildew bacteria, etc., to achieve pollution-free production and reduce tobacco powdery mildew Occurrence, good preventive effect

Active Publication Date: 2017-11-03
GUIZHOU TOBACCO SCI RES INST
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  • Abstract
  • Description
  • Claims
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AI Technical Summary

Problems solved by technology

Because tobacco powdery mildew has the characteristics of high reproduction rate, large genetic variation and high fitness, it is more and more difficult to control it with chemical agents

Method used

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  • Feed Paenibacillus and its application
  • Feed Paenibacillus and its application
  • Feed Paenibacillus and its application

Examples

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Embodiment Construction

[0021] from flue-cured tobacco ( Nicotiana tabacum L.) isolated from rhizosphere soil. Feed Paenibacillus BJF-18 was cultured on NA medium for 24 hours to produce a single colony, which was round, light yellow, viscous, opaque, protruding on the surface, with neat edges, short straight rods, Gram Positive bacteria can produce spores and secrete protease, cellulase, etc.

[0022] The above-mentioned bacterial strain BJF-18 for preventing and controlling tobacco powdery mildew was shaken and cultured in NB culture medium at 30°C and 180rpm for 36-48 hours, then centrifuged at 6000rpm for 10 minutes, and the wet bacteria and bacteria preservation solution of BJF-18 were taken, and the mass-volume ratio was 1 :50 to prepare bacterial agent, the concentration of viable bacteria in the finished bacterial agent is 1×10 10 -1×10 12 CFU / ml, the bacterial cell preservation solution is 0.01 mol / L phosphate buffer solution, and also contains Tween 40 non-ionic surfactant with a conce...

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Abstract

The invention discloses a Paenibacillus pabuli and its application. Its morphological characteristics: Paenibacillus pabuli BJF‑18 (Paenibacillus pabuli) is cultivated on NA medium for 24 hours to produce a single colony, and the colony is round and light yellow. Viscous, opaque, protruding surface, neat edges, short straight rod-shaped bacteria, Gram-positive bacteria, can produce spores, can secrete protease, cellulase, etc. The invention has good control effect on tobacco powdery mildew at seedling stage of flue-cured tobacco, has good preventive effect, and is environmentally friendly.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a Paenibacillus forage, and also relates to the use of the Paenibacillus forage in preventing and treating tobacco powdery mildew at the seedling stage of floating seedling cultivation. Background technique [0002] Tobacco powdery mildew (tobacco white powder) is a very harmful disease in tobacco production, and the pathogenic bacteria are Erysiphe cichoracearum DC, which has a wide range of hosts and is highly harmful, is an airborne fungal disease that occurs throughout the growth period from floating seedlings to flue-cured tobacco. It is most common in South China, Southwest China and Central China, where the rate of diseased plants can reach 100%, the rate of diseased leaves generally ranges from 10% to 20%, and can reach more than 80% in severe cases, causing serious economic losses to tobacco farmers. In the case of intensive floating seedling cultivation, the pathogen spre...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00C12R1/01
CPCA01N63/00C12N1/205C12R2001/01
Inventor 汪汉成王茂胜陈兴江夏海乾陆宁商胜华
Owner GUIZHOU TOBACCO SCI RES INST
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