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Sdr5-fc fusion protein and uses thereof

A fusion protein, sdr5-fc technology, applied in the direction of peptide/protein components, hybrid peptides, recombinant DNA technology, etc., can solve the problems of increasing patient pain and treatment costs, short half-life, etc., to reduce pain and treatment costs, prolong In vivo half-life effects

Inactive Publication Date: 2015-06-17
SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the prior art, the recombinant protein DR5 has a short half-life in the body, often has not reached the optimal therapeutic concentration and the effect has been cleared, and must be administered frequently, which increases the suffering of patients and the cost of treatment

Method used

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  • Sdr5-fc fusion protein and uses thereof
  • Sdr5-fc fusion protein and uses thereof
  • Sdr5-fc fusion protein and uses thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0049] Acquisition of sDR5 gene:

[0050] The RNA of lung cancer cell lines A549, H1299, SPC-A1 and liver cancer cell line HepG2 were extracted respectively, the sDR5 gene was obtained by RT-PCR technology, and the size of the gene was detected by 2% agarose gel electrophoresis. The results are as follows: figure 1 As shown, the size of the obtained sDR5 gene fragment is in line with expectations, and the sequence of the obtained sDR5 gene is shown in SEQ ID NO:2 after sequencing.

Embodiment 2

[0052] Sequence overlap of sDR5-Fc DNA fragment and construction of eukaryotic expression vector:

[0053] By overlapping PCR technology, the sDR5 gene sequence (SEQ ID NO: 2) obtained in Example 1 was overlapped with the Fc sequence (SEQ ID NO: 4) to form a sDR5-Fc DNA fragment (SEQ ID NO: 6);

[0054] The sDR5-Fc DNA fragment and the eukaryotic expression vector pcDNA3.1 (+) were digested with Nhe I and Kpn I respectively, and then the sDR5-Fc DNA fragment was inserted into the eukaryotic expression vector pcDNA3.1 (+) with ligase Between the Nhe I / Kpn I, the recombinant vector pcDNA3.1-sDR5-Fc was obtained, and the results were as follows figure 2 As shown, after sequencing, the nucleotide sequence of the recombinant vector is shown in SEQ ID NO:7.

[0055] Recombinant protein expression:

[0056] The recombinant vector pcDNA3.1-sDR5-Fc obtained in Example 2 was transfected into 293T or CHO cells with liposome transfection reagent jetPRIME. After 48 hours, 10 μl of t...

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Abstract

The present invention belongs to the technical field of biology, and relates to disease treatment, particularly to sDR5-Fc fusion protein and uses thereof. The sDR5-Fc fusion protein comprises an sDR5 functional sequence part and an immunoglobulin Fc region, wherein the sDR5 functional sequence part and the immunoglobulin Fc region are directly connected and / or are connected through a connection fragment. According to the present invention, the natural protein DR5 is adopted to fuse with the antibody Fc fragment, such that the in vivo half-life is prolonged on the base of provision of the biological activity of the functional protein, the frequent administration is not required, and the pain and the treatment cost of patients are reduced.

Description

【Technical field】 [0001] The invention belongs to the field of biotechnology and relates to the treatment of diseases, in particular to a sDR5-Fc fusion protein and its application. 【Background technique】 [0002] Hepatitis is a disease that seriously endangers people's life and health and has a high mortality rate. The etiology mainly includes the destruction of liver parenchymal cells caused by viral infection, excessive alcohol intake and ingestion of chemical poisons. Its pathogenic mechanism is complex, and its pathogenic factors include Viruses, bacteria, parasites, chemical poisons, drugs and poisons, alcohol, etc. Hepatitis can usually be divided into many different types: according to the etiology, it can be divided into viral hepatitis, drug-induced hepatitis, alcoholic hepatitis, toxic hepatitis, etc.; according to the length of the disease, it can be divided into acute hepatitis and chronic hepatitis According to the severity of the disease, chronic hepatitis ca...

Claims

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Application Information

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IPC IPC(8): C07K19/00C12N15/63C12N5/10A61K38/16A61K48/00A61P31/12
Inventor 万晓春田永帅赵琦王蒲阮庆国
Owner SHENZHEN INST OF ADVANCED TECH CHINESE ACAD OF SCI
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