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Preparation process for microbial preparation for repairing nitrotoluene contaminated soil

A technology for microbial preparation and polluted soil, applied in the field of microorganisms, can solve the problems of inability to double as fertilizer, poor treatment effect of dinitrotoluene, slow reproduction rate of strains, etc., and achieves the effects of low cost, increased yield, and simple and feasible process

Active Publication Date: 2015-06-10
青岛新地原生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Chinese patent CN102864108A discloses a composite bacterial agent for repairing nitrotoluene-contaminated soil. The bacterial agent has a certain repairing function, but the reproduction rate of some strains is slow, and the treatment effect on dinitrotoluene is poor, so it cannot be used as a fertilizer

Method used

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  • Preparation process for microbial preparation for repairing nitrotoluene contaminated soil

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] A preparation process for microbial preparations for repairing nitrotoluene-contaminated soil, which includes the following steps: mixing and stirring the composite bacterial liquid and biological organic matter evenly, cultivating at 30°C for 12 hours, then adding auxiliary materials and natural minerals, 100 rpm Min stirring for 3 minutes, and finally low-temperature drying, the drying temperature is 20 ° C, and the water content after drying is 8% (w / w), to get final product; the mass ratio of the composite bacterial liquid, auxiliary materials, natural minerals and biological organic matter is 1 :1:3:10.

[0021] The composite bacteria liquid is prepared according to the following method:

[0022] Acinetobacter baumannii, Bacillus cereus, Rhodococcus erythrococcus, Micrococcus luteus and Enterococcus faecalis were cultured to a concentration of 1×10 8 Bacterial solution of each / m1, according to the volume ratio of 1:1:2:3:3, mix and stir evenly to get final product...

Embodiment 2

[0031] A preparation process for microbial preparations for repairing nitrotoluene-contaminated soil, which includes the following steps: mixing and stirring the composite bacterial liquid and biological organic matter evenly, cultivating at 30°C for 12 hours, then adding auxiliary materials and natural minerals, 100 rpm min stirring for 3min, and finally carry out low-temperature drying, the drying temperature is 20 ° C, and the water content after drying is 8% (w / w), to obtain final product; the mass ratio of the composite bacterial liquid, auxiliary materials, natural minerals and biological organic matter is 2 :2:4:15;

[0032] The composite bacteria liquid is prepared according to the following method:

[0033] Acinetobacter baumannii, Bacillus cereus, Rhodococcus erythrococcus, Micrococcus luteus and Enterococcus faecalis were cultured to a concentration of 1×10 8 Bacterial liquid of each / ml, according to the volume ratio of 1: 1: 2: 3: 3, mix and stir evenly to get fin...

Embodiment 3

[0046] The present invention restores polluted soil test:

[0047] Adopt the microbial preparation prepared by the embodiment of the present invention 2 process to process the polluted soil near a certain explosives factory, choose the soil area to be 1 mu of land, at first the soil is plowed and leveled, the depth of plowing is 40cm, spray water, and keep the soil moisture content to be 30 %, then spread the microbial preparation according to the addition of 200g of microbial preparation per cubic meter of soil, and after one week, detect the content of nitrobenzene, see Table 1:

[0048] Table 1

[0049] indicators

[0050] Conclusion: After testing, it is found that the microbial preparation prepared by the process of the present invention can effectively remove nitrotoluene substances.

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Abstract

The invention belongs to the technical field of microorganisms and discloses a preparation process for a microbial preparation for repairing nitrotoluene contaminated soil. The preparation process comprises the following steps: mixing and uniformly mixing a compound bacterial solution with biomass organic substances, culturing for 12 hours at 30 DEG C, adding auxiliary materials and natural minerals, and stirring; finally, drying at a low temperature to obtain the microbial preparation. The preparation process disclosed by the invention is simple and feasible; the prepared microbial preparation can be used for effectively treating nitrotoluene contaminated soil, is good in treatment effect and low in cost.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a preparation process of a microbial preparation for repairing nitrotoluene-contaminated soil. Background technique [0002] TNT (2,4,6-trinitrotoluene) is a colorless or light yellow crystal with a melting point of 354K (80.9°C). TNT is currently the production explosive with the highest output. In recent years, the manufacture, processing and packaging of TNT in ammunition workshops have caused highly concentrated pollution of soil, which will eventually lead to pollution of groundwater. Long-term exposure to nitrotoluene can increase the chances of suffering from anemia and abnormal liver function. Blood and liver effects, enlarged spleens, and other adverse effects on the immune system were also found in animals injected or inhaled. There is also evidence that TNT has adverse effects on male reproductive function, and TNT is also listed as a possible carc...

Claims

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Application Information

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IPC IPC(8): C12N1/20B09C1/10C12R1/085C12R1/265C12R1/01
CPCB09C1/10C12N1/20
Inventor 李丽萍何颖霞
Owner 青岛新地原生物科技有限公司
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