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A Method for Batch Freezing Grouper Sperm

A grouper and sperm technology, applied in the preservation, application, and animal husbandry of human or animal bodies, can solve problems such as low efficiency, complicated operation, and small quantity, and achieve high efficiency, ensure fertilization rate, and reduce workload Effect

Active Publication Date: 2016-08-24
HAINAN UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of the present invention is to provide a method for fast batch preservation of grouper sperm, which solves the problems of low efficiency, small quantity and complicated operation of the existing method for cryopreservation of grouper sperm

Method used

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  • A Method for Batch Freezing Grouper Sperm
  • A Method for Batch Freezing Grouper Sperm
  • A Method for Batch Freezing Grouper Sperm

Examples

Experimental program
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Embodiment Construction

[0029] The present invention will be further explained below in conjunction with specific examples of batch fast freezing preservation of grouper spermatozoa.

[0030] 1. Collection of qualified semen: in July in Lingshui, Hainan, healthy and mature male grouper fish were selected for semen collection. Before semen collection, the fish body was dried and urine and feces were removed. 50ml EP tubes made of paper, sealed and quickly placed in an ice bath sample box for storage and transportation. A small amount of sperm samples were placed under a microscope for seawater activation and observed. At room temperature, 80% of the sperm remained in a moving state within 5 minutes as qualified samples. ( image 3 Shown, the effect of different sperm collection periods on the cryopreservation viability)

[0031] 2) Dilution of sperm: select the qualified sperm of step 1), adopt conventional Ts-2 (Tris acid 10mmol / L, KHCO 3 100mmol / L, sucrose 110mmol / L, fetal bovine serum 10%) is sp...

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Abstract

The invention discloses a method for freezing garrupa sperms in batch. The method includes the steps of sperm collecting, sperm and antifreeze protection liquid mixing, split charging, freezing and the like. According to the method, the problems that time and labor are wasted for freezing a small number of the sperms, and the actual production requirement cannot be met exist, and a liquid nitrogen specimen holder is adopted for rapidly freezing the garrupa sperms in batch. By means of the method, hundreds of milliliters of the garrupa sperms can be rapidly frozen, the average vigor can be higher than 90% after unfreezing is carried out, and the problem that a large number of sperms are needed for manually reproducing garrupa is solved.

Description

technical field [0001] The invention relates to the field of fish reproduction and breeding, in particular to a method for rapidly preserving grouper sperm in batches. Background technique [0002] The ultra-low temperature preservation of fish semen is of great significance in aquaculture, genetic breeding and germplasm resource preservation: the ultra-low temperature cryopreservation of fish semen can not only realize the long-distance transportation of germplasm materials, but also facilitate the hybridization of fish in different regions, Breeding, obtaining excellent traits and protecting genetic diversity; it can also provide long-term and stable materials for aquaculture and experimental biology research. Research on cryopreservation of fish sperm began in the early 1950s. British scholar Blaxter used dry ice to directly freeze the Pacific herring (Clupea pallasi) testis, and obtained a fertilization rate of 80%. After that, scholars at home and abroad have carried ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 骆剑陈国华王小刚吴长松王茜
Owner HAINAN UNIVERSITY
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