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Tissue culture rapid propagation method of liquorice

A technology of tissue culture and licorice, which is applied in the field of plant tissue culture, can solve the problems of in vitro rapid propagation of licorice that have not yet been seen.

Inactive Publication Date: 2015-06-10
陈桂容
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there have been no reports about rapid propagation of licorice in vitro, which seriously affects the development of licorice industrialization.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] (1) Treatment of explants: Select the stems with axillary buds from strong licorice plants as explants, cut off the leaves, cut into 3.0-5.0 long segments with 1-2 axillary buds, and wash them with washing powder first. Rinse with water for 2 hours, then gently scrub the surface of the explants with a soft brush to remove the dust and some bacteria on the surface, then rinse with tap water for 1 hour and place it in an ultra-clean workbench, first disinfect it with 75% ethanol for 5 seconds, and then use sterile Wash 3 times with sterile water, then disinfect with 0.1% mercuric chloride solution for 5 minutes, rinse 4 times with sterile water, and dry the water drops on the surface with sterile filter paper before use.

[0018] (2) Tufted bud guide: cut the stems with axillary buds treated in step (1) into 1.5-2.5 cm pieces and inoculate them on the induction medium, culture them in the dark at 28°C for 3 days, and then place them in the Illuminate for 11 hours, and cul...

Embodiment 2

[0023] (1) Treatment of explants: Select the stems with axillary buds from strong licorice plants as explants, cut off the leaves, cut into 3.0-5.0 long segments with 1-2 axillary buds, and wash them with washing powder first. Rinse with water for 3 hours, then gently scrub the surface of the explants with a soft brush to remove the dust and some bacteria on the surface, then rinse with tap water for 2 hours, then place them in an ultra-clean workbench, first sterilize with 75% ethanol for 10 seconds, and then use sterile Wash 5 times with sterile water, then disinfect with 0.1% mercuric chloride solution for 7 minutes, rinse 5 times with sterile water, and dry the water droplets on the surface with sterile filter paper before use.

[0024] (2) Tufted bud guide: cut the stems with axillary buds treated in step (1) into 1.5-2.5cm pieces and inoculate them on the induction medium, culture them in the dark at 28°C for 4 days, and then place them in the Illuminated for 12 hours, a...

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Abstract

The invention discloses a tissue culture rapid propagation method of liquorice. The axillary bud stems of the liquorice are taken as the explants to establish a liquorice in-vitro rapid propagation system, and a foundation is laid for the large-scale production of the liquorice. The stem with the axillary buds is taken as the explant, and the tissue culture rapid propagation of the liquorice is realized by use of the processes of multiple shoot induction, multiplication culture, root and shoot induction, test-tube plantlet acclimatization and transplant and the like; the technical support is provided for the industrialization development of the liquorice.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a rapid propagation method for tissue culture of licorice. Background technique [0002] Licorice ( Glycyrrhiza uralensis ) is a traditional Chinese medicinal material in my country, and it is also an important solid-sparing plant in the desert and semi-desert areas of western my country. Coumarins, a variety of alkaloids, etc., have anti-inflammatory, antibacterial, anti-tumor, anti-viral and other effects. The traditional method of obtaining licorice is to dig wild plant resources, resulting in soil erosion and severe soil desertification. In addition, licorice seeds mainly come from wild licorice, whose varieties are mixed, seeds are scarce, and germination is extremely low. Wild licorice resources can no longer meet the needs of large-scale and standardized production, which has become the biggest bottleneck hindering the development of l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 陈桂容
Owner 陈桂容
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