Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for constructing regeneration system of Magnolia officinalissubsp. biloba(Rehd. et Wils.) Law.

A kind of Magnolia officinalis and systemic technology, applied in the field of plant tissue culture, can solve the problems such as the unformed industrialized production mechanism, low natural reproduction rate, and difficulty in tissue culture, and achieve the effect of promoting the commercialization process

Inactive Publication Date: 2015-06-10
梁仕华
View PDF3 Cites 10 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] At present, Magnolia officinale mainly relies on seed propagation for seedling production, which has problems such as long propagation cycle, low efficiency, and low reproduction coefficient, and a sound industrial production mechanism has not yet been formed.
In recent years, due to the gradual increase in the demand for Magnolia officinalis, wild resources have gradually decreased, and its seeds themselves have the characteristics of deep dormancy, so the natural reproduction rate is generally low
In addition, as a relatively primitive woody plant in the Magnoliaceae, Magnolia officinale is more difficult to tissue culture.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0020] (1) Establishment of a sterile system: Select mature, plump, complete and pest-free Magnolia officinalis seeds, and preliminarily wash them under tap water. Seeds were soaked in 0.1% potassium permanganate solution for 2 hours until the color of potassium permanganate solution became lighter to pink, rinsed with tap water, then sterilized in 75% ethanol solution for 5 minutes, rinsed with sterile water 4 times, and sterile filter paper Drain, manually remove the outer seed coat, and inoculate it on the germination medium to start the culture. The germination medium is 3 / 4B 5 + 0.3mg / L 6-BA+0.2mg / L NAA +3.5% sucrose+0.5% agar+0.1% activated carbon, the pH value is 5.8.

[0021] (2) Primary culture: Inoculate the aseptic seedlings with germinated cotyledons that have not fallen off from the aseptic system on the primary medium for cultivation. After inoculation, first culture in total darkness at 28°C for 10 days, and then place in light for 15 hours a day , the ligh...

Embodiment 2

[0027] (1) Establishment of a sterile system: Select mature, plump, complete and pest-free Magnolia officinalis seeds, and preliminarily wash them under tap water. Seeds were soaked in 0.3% potassium permanganate solution for 4 hours until the color of potassium permanganate solution became lighter to pink, rinsed with tap water, then sterilized in 75% ethanol solution for 5 minutes, rinsed with sterile water for 5 times, and washed with sterile filter paper. Drain, manually remove the outer seed coat, and inoculate it on the germination medium to start the culture. The germination medium is 3 / 4B 5 + 0.5mg / L 6-BA+0.4mg / L NAA +2.5% sucrose+0.5% agar+0.1% activated carbon, the pH value is 5.6.

[0028] (2) Primary culture: Inoculate the aseptic seedlings with germinated cotyledons that have not fallen off from the aseptic system on the primary medium for cultivation. After inoculation, first culture in total darkness at 28°C for 10 days, and then place in light for 13 hours a...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for constructing a regeneration system of Magnolia officinalissubsp. biloba(Rehd. et Wils.) Law., and relates to a tissue culture and rapid propagation method of Magnolia officinalissubsp. biloba(Rehd. et Wils.) Law.. According to the method disclosed by the invention, Magnolia officinalissubsp. biloba(Rehd. et Wils.) Law. is taken as an initial explant, the regeneration system of the Magnolia officinalissubsp. biloba(Rehd. et Wils.) Law. is established through processes of sterile system establishment, primary culture, multi-shoot propagation culture, multi-shoot strong seedling culture, test tube seedling rooting, test tube acclimatizating and transplanting, and the like, and a production method is provided for rapid regeneration of excellent Magnolia officinalissubsp. biloba(Rehd. et Wils.) Law. seed sources.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for constructing a Magnolia officinalis regeneration system. Background technique [0002] Magnolia officinalis ( Magnolia officinalis subsp. biloba (Rehd. et Wils.) Law.)) also known as Lushan Magnolia officinalis, is a subspecies of Magnolia officinalis. It is a unique economic tree species in my country and a second-class national key protected plant. It is a rare and endangered plant integrating medicinal, timber and ornamental. It is mainly distributed in Hunan, Hubei, Guangxi and other places. The root bark, bark, flowers, buds and seeds of Magnolia officinalis can be used as medicine. It has antibacterial, anti-ulcer, muscle relaxation, anti-spasmodic and anti-allergic effects. It is a commonly used traditional Chinese medicine for gastrointestinal diseases. [0003] At present, Magnolia officinale mainly relies on seed pr...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 梁仕华
Owner 梁仕华
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com