Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Building method of aristolochia cinnabarina regeneration system

A cinnabar lotus and system technology, applied in the field of plant tissue culture, can solve the problems of unformed industrialized production mechanism, long breeding cycle, low reproduction coefficient, etc., and achieve the effect of promoting the commercialization process

Inactive Publication Date: 2019-01-22
林登淞
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, cinnabar lotus mainly relies on seed propagation for seedling production, which has problems such as long reproduction cycle, low efficiency, and low reproduction coefficient, and a sound industrial production mechanism has not yet been formed.
In recent years, due to the gradual increase in demand for cinnabar lotus, wild resources have gradually decreased, and the seeds themselves have the characteristics of deep dormancy, so the natural reproduction rate is generally low
In addition, as a relatively primitive woody plant of Magnoliaceae, cinnabar lotus is difficult to cultivate in tissue culture.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0014] (1) Establishment of a sterile system: select mature, plump, complete and pest-free Cinnabar chinensis seeds, and preliminarily wash them under tap water. Seeds were soaked in 0.1% potassium permanganate solution for 5 hours until the color of potassium permanganate solution became lighter to pink, rinsed with tap water, then sterilized in 75% ethanol solution for 8 minutes, rinsed with sterile water for 7 times, and washed with sterile filter paper. Drain, manually remove the outer seed coat, and inoculate it on the germination medium to start the culture. The germination medium is 3 / 4B 5 + 0.5mg / L 6-BA+0.4mg / L NAA +3.6% sucrose+0.5% agar+0.2% activated carbon, the pH value is 5.7.

[0015] (2) Primary culture: Inoculate the aseptic seedlings with germinated cotyledons that have not fallen off from the aseptic system on the primary medium for cultivation. After inoculation, culture in total darkness at 24°C for 12 days, and then place in light for 16 hours a day , t...

Embodiment 2

[0021] (1) Establishment of a sterile system: select mature, plump, complete and pest-free Cinnabar chinensis seeds, and preliminarily wash them under tap water. Seeds were soaked in 0.3% potassium permanganate solution for 4 hours until the color of potassium permanganate solution became lighter to pink, rinsed with tap water, then sterilized in 75% ethanol solution for 6 minutes, rinsed with sterile water for 6 times, and washed with sterile filter paper. Drain, manually remove the outer seed coat, and inoculate it on the germination medium to start the culture. The germination medium is 3 / 4B 5 + 0.6mg / L 6-BA+0.5mg / L NAA +2.8% sucrose+0.6% agar+0.2% activated carbon, the pH value is 5.5.

[0022] (2) Primary culture: Inoculate the aseptic seedlings with germinated cotyledons that have not fallen off from the sterile system to the primary medium for cultivation. After inoculation, first culture in total darkness at 24°C for 12 days, and then place in light for 15 hours a da...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a building method of an aristolochia cinnabarina regeneration system, and relates to a tissue culture and rapid propagation method of aristolochia cinnabarina. The building method of the aristolochia cinnabarina regeneration system is characterized by taking aristolochia cinnabarina as a primary explant and building the regeneration system of aristolochia cinnabarina through processes of building an aseptic system, carrying out primary culture, carrying out proliferation culture on cluster buds, culturing strong seedlings of the cluster buds, carrying out rooting on test-tube seedlings, acclimatizing and transplanting the test-tube seedlings, and the like. A production method is provided for rapid propagation of high-quality seed resources of aristolochia cinnabarina.

Description

technical field [0001] The invention relates to a method for plant tissue culture in agricultural biotechnology, in particular to a method for constructing a cinnabar lotus regeneration system. Background technique [0002] Chuanzhushalian is a plant of Aristolochiaceae. Its roots are dug in late spring, fibrous roots are removed, and dried. The smell is bitter. Has heat-clearing and toxic substances removing, analgesic. For toothache, sore throat, stomachache. At present, the production of seedlings of cinnabar lotus mainly relies on seed propagation, which has problems such as long reproduction cycle, low efficiency, and low reproduction coefficient, and a sound industrial production mechanism has not yet been formed. In recent years, due to the gradual increase in demand for cinnabar lotus and the gradual decrease in wild resources, the seeds themselves have the characteristics of deep dormancy, and the natural reproduction rate is generally low. In addition, as a rel...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 林登淞
Owner 林登淞
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com