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Method for catalyzing synthesis of vitamin A palmitate by immobilized esterase e.coli BioH

A technology of palmitate and vitamin, applied in fermentation and other directions, can solve problems such as thermal stability effects, and achieve the effects of good stability, mild reaction conditions and lower use costs

Active Publication Date: 2018-01-09
SHANGYU NHU BIOCHEM IND +3
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method uses halogen raw materials and pyridine in the reaction process, the reaction conditions are milder than the acid chloride method, and the yield can reach 95%, but the product has a great influence on the thermal stability of the product due to the residue of halogen ions

Method used

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  • Method for catalyzing synthesis of vitamin A palmitate by immobilized esterase e.coli BioH

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] The synthesis of embodiment 1 vitamin A alcohol

[0027] In a 1000ml four-neck flask, add 30g of vitamin A acetate and 400ml of ethanol, then add 6g of immobilized esterase E.coli BioH (the weight content of esterase E.coli BioH is 15%). The reaction was carried out for 18 hours under the MPa vacuum distillation condition, and the reaction liquid sampling was detected by HPLC, and the residual vitamin acetate was 0.43%.

Embodiment 2

[0028] The synthesis of embodiment 2 vitamin A alcohol

[0029] In a 1000ml four-neck flask, add 30g of vitamin A acetate and 400ml of ethanol, then add 18g of immobilized esterase E.coli BioH (the weight content of esterase E.coli BioH is 20%). The reaction was carried out for 10 hours under the MPa vacuum distillation condition, and the reaction liquid was sampled and detected by HPLC, and the residual vitamin acetate was 0.39%.

Embodiment 3

[0030] The synthesis of embodiment 3 vitamin A alcohol

[0031]In a 1000ml four-neck flask, add 30g of vitamin A acetate and 600ml of methanol, then add 18g of immobilized esterase E.coli BioH (the weight content of esterase E.coli BioH is 20%), at 20°C, -0.08 The reaction was carried out under MPa vacuum distillation conditions for 16 hours, and the reaction liquid was sampled and detected by HPLC, and the vitamin acetate residue was 0.21%.

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Abstract

The invention uses immobilized esterase E.coli BioH to catalyze the synthesis of vitamin A palmitate in a non-aqueous phase system. In this method, vitamin A acetate and lower alcohols are used as raw materials, which are first converted into vitamin A alcohol under the catalysis of immobilized esterase E. Catalyzed esterification reaction to obtain vitamin A palmitate. The conversion rate of the method is as high as 98%, and the number of times of applying the enzyme can reach more than 40 times. The subsequent separation and purification of the product is simple, and the production cost is low, which is conducive to the realization of industrialization. The vitamin A palmitate prepared by the method of the present invention has a light yellow oily appearance. The method of USP28 is used for analysis, and the potency is 1.79-1.8 million IU / g, which is equivalent to 98%-99% of the content. It can be widely used as medicine, feed additive, food additive, etc.

Description

technical field [0001] The invention relates to a method for synthesizing vitamin A palmitate, in particular to a method for catalyzing the synthesis of vitamin A palmitate by immobilized enzymes. Background technique [0002] Vitamin A palmitate has been widely used as medicine, food additive and feed additive, etc. There have been reports on various synthetic methods, including the preparation of vitamin A palmitic acid from vitamin A alcohol or lower fatty acid esters of vitamin A The ester method involves a variety of reaction routes. [0003] The synthetic route disclosed in Japanese Patent JP 62,248495 is: vitamin A acetate and palmitic acid are obtained under the action of lipase to obtain vitamin A palmitate; the synthetic route disclosed in British Patent GB 816224 is: vitamin A acetate and palmitic acid alkyl Vitamin A palmitate is obtained under the action of sodium methylate; British Patent GB 1293041 discloses a synthetic route: vitamin A alcohol and palmitoyl ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P23/00
Inventor 于洪巍覃广德吕国锋陈志荣刘浩黄国东解丕昭
Owner SHANGYU NHU BIOCHEM IND
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