Automatic reaction device and method for pretreating cells

Abstract

The invention discloses automatic reaction device and method for pretreating cells. The device comprises a reaction box body, a base, a box cover and a piston, wherein a middle cavity is formed in the center of the reaction box body; seven cavities are formed in the periphery of the hollow cavity; seven square cavities are correspondingly formed between the middle cavity and the seven cavities in the periphery; a control valve is put into each square cavity; communication between an inner chamber and an outer chamber is controlled through up and down movement; the lower part of the reaction box body is tightly connected with the base; the box cover is arranged at the upper part of the reaction box body; a piston rod which is matched with the middle cavity penetrates through the box cover; and the outer side of a second PCR reagent cavity is connected with a PCR chip for detecting nucleic acid. According to the reaction device and method, crushing and cracking of original samples, and purifying and extracting of the nucleic acid and PCR amplification are integrated in a reaction box; the operation process is simplified; the pretreatment efficiency is greatly improved; meanwhile, the reaction volume is reduced; the portability of an instrument is improved; and a foundation is laid for rapid, safe and accurate nucleic acid detection.

Description

【Technical field】 [0001] The invention relates to the technical field of cell analysis, in particular to an automatic reaction device and method for pretreating cells. 【Background technique】 [0002] Nucleic acid detection is the most eye-catching field in the post-genome era, and it will have an immeasurable impact on genetics, clinical medicine, and molecular diagnostics. In order to complete high-quality nucleic acid detection, cells need to be pretreated quickly and efficiently to extract high-purity nucleic acids. Cell pretreatment mainly includes three steps of cell lysis, nucleic acid purification and PCR amplification, aiming to purify and proliferate the nucleic acid in the cells after pulverizing the cells to achieve the purity and concentration required for detection. [0003] At present, the pretreatment of cells on the market generally adopts an extension operation, that is, each step of the operation is completed on multiple instruments, which will not only ma...

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Problems solved by technology

[0004] The purpose of the present invention is to provide an automatic reaction device and method for pretreatment of cells to solve the complex, bulky and cumbersome problems of the existing cell pretreatment system; Crushing and lysis, purification and extraction of nucleic acid, and PCR amplification are integrated in one reaction box, which realizes the integrated operation of cell pretreatment; not only simplifies the operation process, but also greatly improves the pretreatment efficiency; at the same time reduces the reaction volume and improves It improves the portability of the instrument and lays the foundation for fast, safe and accurate nucleic acid detection

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