Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

A kind of micromonospora fermentation medium and culture method for producing gentamicin

A fermentation medium and gentamicin technology, applied in the field of fermentation, can solve the problems of affecting the quality of fermentation broth and fermentation effect, high production cost of gentamicin, and reduced extraction yield, etc., to improve the quality and fermentation technology level , Improving the level of fermentation technology and avoiding the effect of unfavorable factors

Active Publication Date: 2018-04-20
宁夏泰瑞制药股份有限公司
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] 1 The fermentation production process has not changed much, and the fermentation unit is maintained at about 1500u / ml, resulting in a low fermentation yield
[0004] 2 The quality of the animal nitrogen source contained in the fermentation medium affects the quality of the fermentation broth and the fermentation effect
At present, corn gluten meal or fish meal or both are added to the medium for domestic fermentation production of gentamicin, resulting in more residual protein in the late stage of fermentation, higher viscosity of the fermentation broth, and reduced extraction yield
[0005] 3 The production cost of gentamicin is relatively high, and the cost of nitrogen source accounts for about 30% of the fermentation cost

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • A kind of micromonospora fermentation medium and culture method for producing gentamicin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0063] primary seed culture

[0064] at 1m 3 Add 10L of maltose, 20kg of mixed starch, 1L of soybean oil, 25kg of low-temperature pressed soybean cake powder, 15kg of rice distiller's grains, 5kg of corn gluten powder, 2kg of light calcium carbonate, 1kg of ammonium sulfate, and 10g of maltase into the primary seed tank. Firstly, the primary seed culture medium is sterilized, cooled, and pressurized with sterile air, and then under flame protection, the cultured micromonospora mother bottle fermentation liquid is connected to the primary seed tank for cultivation, and the inoculum size is 0.5L. During the primary seed cultivation process, tank pressure is 0.04-0.08MPa; tank temperature is 34-37°C; air flow: 0-10h, 50m 3 / h; 6h~transplantation: 80m 3 / h; stirring speed 60r / min; pH7~8; culture time 50h. After the primary seed cultivation is completed, the cell concentration is 16%; the pH value is 7.1, and there is no other bacterial contamination.

[0065] secondary seed c...

Embodiment 2

[0071] primary seed culture

[0072] at 1m 3 Add 12L of maltose, 22kg of mixed starch, 2L of corn oil, 27kg of low-temperature pressed soybean cake powder, 16kg of rice distiller's grains, 6kg of corn gluten powder, 2.5kg of light calcium carbonate, 2kg of ammonium sulfate, and 15g of maltase into the primary seed tank. Firstly, the primary seed culture medium is sterilized, cooled, and pressurized with sterile air, and then under flame protection, the cultured micromonospora mother bottle fermentation liquid is connected to the primary seed tank for cultivation, and the inoculum size is 0.6L. During the primary seed cultivation process, tank pressure is 0.04-0.08MPa; tank temperature is 34-37°C; air flow: 0-10h, 52m 3 / h; 6h~transplantation: 85m 3 / h; stirring speed 70r / min; pH7~8; culture time 51h. After the primary seed cultivation is over, the cell concentration is 17%; the pH value is 7.3, and there is no other bacteria contamination.

[0073] secondary seed culture ...

Embodiment 3

[0079] primary seed culture

[0080] at 1m 3 Add 15L of maltose, 25kg of mixed starch, 3L of soybean oil, 30kg of low-temperature pressed soybean cake powder, 17kg of rice distiller's grains, 7kg of corn gluten powder, 3kg of light calcium carbonate, 3kg of ammonium sulfate, and 20g of maltase into the primary seed tank. Firstly, the primary seed culture medium is sterilized, cooled, and pressurized with sterile air, and then under flame protection, the cultured micromonospora mother bottle fermentation liquid is connected to the primary seed tank for cultivation, and the inoculum size is 0.7L. During the primary seed cultivation process, tank pressure is 0.04-0.08MPa; tank temperature is 34-37°C; air flow: 0-10h, 55m 3 / h; 6h~transplantation: 90m 3 / h; stirring speed 80r / min; pH7~8; culture time 52h. After the primary seed cultivation is completed, the cell concentration is 20%; the pH value is 7.5, and there is no other bacterial contamination.

[0081] secondary seed c...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The present invention relates to a culture medium and culture method for Micromonospora fermentative production of gentamicin, comprising primary seed medium, secondary seed medium and fermentation medium, characterized in that the culture contains rice distiller's grains and low temperature Pressed soybean cake powder; fermentation culture contains rice distiller's grains, low-temperature pressed soybean cake powder, weakly acidic cation exchange resin and nonionic surfactant. The invention solves the cost problem of raw and auxiliary materials, and minimizes the source of raw and auxiliary materials from being affected by the environment, ensures sufficient supply, and realizes stable and efficient production of gentamicin. unit.

Description

technical field [0001] The invention belongs to the technical field of fermentation, and in particular relates to a culture medium and a culture method for Micromonospora fermentation to produce gentamicin. Background technique [0002] Gentamicin is an aminoglycoside broad-spectrum antibiotic produced by the fermentation of Actinomycetes Micromonospora. It was first discovered by American Weinstein in 1963 and used clinically in 1969. One of the main antibacterial drugs. At present, the domestic use of Micromonospora fermentation to produce gentamicin, the main problems are: [0003] 1 The fermentation production process has not changed much, and the fermentation unit is maintained at about 1500u / ml, resulting in a low fermentation yield. [0004] 2 The quality of animal nitrogen source contained in fermentation medium affects the quality of fermentation broth and fermentation effect. At present, corn gluten meal or fish meal or both are added to the medium for domestic ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/50C12R1/29
Inventor 任勇
Owner 宁夏泰瑞制药股份有限公司
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com