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A method of irradiation pretreatment and batch feeding to realize enzymatic hydrolysis of lignocellulose with high substrate concentration

A lignocellulose and cellulase technology, applied in the direction of fermentation, can solve the problems of unfavorable system material transfer and heat transfer, increased system viscosity, low enzymatic hydrolysis efficiency, etc., and achieves reduction of ethanol distillation energy consumption and no material loss. , the effect of simple operation

Active Publication Date: 2018-05-18
HUNAN AGRICULTURAL UNIV
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Problems solved by technology

Under normal circumstances, the ethanol concentration of batch fermentation will not exceed 40g / L, and when the ethanol concentration in the fermentation broth is lower than 4%, the energy consumption for subsequent ethanol concentration and rectification will increase significantly
Increasing the substrate concentration of the enzymatic hydrolysis system is a direct way to obtain high concentrations of reducing sugars, but high substrate concentrations often lead to increased viscosity of the system, which is not conducive to the material transfer and heat transfer of the system, resulting in low enzymatic hydrolysis efficiency

Method used

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  • A method of irradiation pretreatment and batch feeding to realize enzymatic hydrolysis of lignocellulose with high substrate concentration
  • A method of irradiation pretreatment and batch feeding to realize enzymatic hydrolysis of lignocellulose with high substrate concentration
  • A method of irradiation pretreatment and batch feeding to realize enzymatic hydrolysis of lignocellulose with high substrate concentration

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Experimental program
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Effect test

Embodiment 1

[0024] Effects of Radiation Irradiation on Biochemical Components of Lignocellulose and Sugar Production by Enzymatic Hydrolysis

[0025] 1. The effect of radiation irradiation on the biochemical components of lignocellulose

[0026] Irradiation treatment can directly break the molecular chain of lignocellulose, reduce its degree of polymerization, and realize the degradation of lignocellulose. Lignocellulosic materials are sun-dried or oven-dried, with a moisture content of ≤10%, bundled and placed in an irradiation box, and used under experimental conditions. 60 Co-γ rays are irradiated for pretreatment. The irradiation dose rate is 2kGy / h, and the treatment dose is 400-1200kGy. The lignocellulosic material pretreated by ray irradiation is crushed through a 20-mesh sieve to obtain lignocellulose raw material. Such as figure 1 As shown in the results of changes in the content of biochemical components in rice straw and corn stalks, the content of cellulose, hemicellulose an...

Embodiment 2

[0030] Optimization of Initial Conditions for Batch Enzymatic Hydrolysis (i.e. Adding Feedstock and Enzyme at One Time)

[0031] The key factors affecting the enzymatic hydrolysis efficiency of lignocellulose include initial substrate concentration, enzyme addition amount, hydrolysis time and substrate structural properties. Add the corn stalk raw material treated with a radiation dose of 1200kGy to 0.05M acetic acid-sodium acetate buffer (pH4.8), add 45FPU / g substrate cellulase ( CTec2, Novozymes, Denmark), under the conditions of pH 4.8, temperature 50°C and rotation speed 160rpm, enzymatic hydrolysis for 72 hours, the concentration of reducing sugar in the enzymatic hydrolyzate was determined by DNS method, and the initial conditions of batch enzymatic hydrolysis were optimized. Firstly, the effect of initial substrate concentration on feed saccharification was investigated, starting from image 3 It can be seen that when the initial substrate concentration is 6%, 12%, a...

Embodiment 3

[0034] Fed-batch and batch-add cellulase hydrolysis

[0035] Obtaining high-concentration reducing sugar hydrolyzate of lignocellulosic raw materials is a necessary condition to realize its high-concentration fermentation and reduce the production cost of cellulosic ethanol. In order to obtain high-concentration reducing sugar enzyme hydrolyzate, the fed-batch saccharification strategy of corn stover pretreated by radiation irradiation was studied under the optimized conditions of batch enzyme hydrolysis test. Add corn stalk 6% (wt) at 12h and 36h, add 45FPU per gram of added corn stalk substrate, the substrate concentration of final treatment is 30%, enzymatic hydrolysis reaction 144h, such as Figure 5 As shown, the final reducing sugar concentration obtained is 152.1 g / L, which is 58.4% higher than that in the batch enzyme hydrolyzate with a substrate concentration of 18%.

[0036] In order to further increase the reducing sugar concentration in the enzymatic hydrolyzate, ...

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Abstract

The invention discloses a method for realizing enzymatic hydrolysis of lignocellulose with high substrate concentration through irradiation pretreatment and feeding in batches. It uses the lignocellulosic raw material pretreated by high-dose ray irradiation as the substrate, and adopts the strategy of feeding in batches and adding cellulase in batches to increase the substrate concentration in the enzymatic hydrolysis system, so as to obtain high-concentration reducing sugar. Hydrolyzate. The advantage of the present invention is that the ray irradiation pretreatment technology has the advantages of simple operation, clean and pollution-free, can be carried out under normal temperature and pressure, no material loss, and remarkable effect, and adopts the strategy of feeding in batches to greatly improve the enzymatic hydrolysis system. The concentration of the substrate in the medium can make the concentration of the treated substrate reach 36%. While increasing the concentration of the enzymatic hydrolysis raw material, a high concentration of reducing sugar can be obtained. The high concentration of reducing sugar in the obtained enzymatic hydrolyzate can reach more than 200g / L, which has reached the advanced level at home and abroad. Level. The obtaining of high-concentration reducing sugar enzyme hydrolyzate of lignocellulose can improve the high-concentration ethanol fermentation in the later stage and reduce the energy consumption of ethanol distillation, thereby reducing the production cost of fiber fuel ethanol.

Description

technical field [0001] The invention belongs to the technical field of enzymatic hydrolysis of lignocellulose, in particular to a high substrate concentration enzymatic hydrolysis method for ray irradiation pretreatment of lignocellulose. Background technique [0002] With the depletion of fossil energy, renewable biomass energy has attracted more and more attention. The second-generation fuel ethanol based on lignocellulose is considered to be an important development direction of fuel ethanol. Lignocellulosic feedstock is one of the most abundant renewable energy sources on earth. A large number of crop straws such as rape stalks, corn stalks, and rice straws are discarded or burned every year in the world, which not only causes waste of resources, but also causes environmental pollution. The production of fuel ethanol from lignocellulosic raw materials has the characteristics of renewability and non-pollution. Its recycling can improve air quality, promote economic deve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/14
Inventor 胡秋龙熊兴耀苏小军王克勤陈亮李清明王康
Owner HUNAN AGRICULTURAL UNIV
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