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Method for fermentation production of arachidonic acid by adopting two-stage pH control

An arachidonic acid, stage technology, applied in the field of bioengineering, to achieve the effect of improving utilization rate, shortening fermentation cycle, and simple fermentation strategy

Inactive Publication Date: 2015-05-27
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
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Problems solved by technology

However, how to increase the production of arachidonic acid still needs to be studied urgently

Method used

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  • Method for fermentation production of arachidonic acid by adopting two-stage pH control
  • Method for fermentation production of arachidonic acid by adopting two-stage pH control

Examples

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Embodiment 1

[0036] Example 1 Two-stage pH control strategy accumulates arachidonic acid

[0037] The fermentation method and conditions are the same as the control example 1: NaOH / HCl, ammonia water, ammonia gas, ammonium nitrate or ammonium hydroxide are used to control the pH of the initial fermentation stage to 4-6 except for the following changes; During the accumulation period (48h), use NaOH / HCl, ammonia, ammonia, ammonium nitrate or ammonium hydroxide to control and adjust the pH to 5-8, and maintain a stable pH until the end of the fermentation. After fermentation, the biomass, oil content and arachidonic acid content were measured.

[0038] The method for determining the biomass of each of the above examples is: vacuum filtration of the fermentation broth, washing with distilled water 3 times, drying the wet bacteria in an oven at 60° C. overnight, and weighing. The calculation formula of biomass is:

[0039]

[0040] Oil extraction method: Weigh a certain quality of dried bacteria, g...

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Abstract

The invention discloses a method for fermentation production of arachidonic acid by adopting two-stage pH control. According to the method, mortierella alpine is taken as a production strain; a two-stage pH control strategy is adopted; the pH of a fermentation solution is regulated to be 4 to 6 in an early stage of fermentation; after the strain is transformed from a growth type to a grease accumulation type, the pH of the fermentation solution is regulated to 5 to 8 for fermentation culture. By adopting the method disclosed by the invention, the yield of the arachidonic acid can be remarkably increased.

Description

Technical field [0001] The invention belongs to the field of bioengineering, and relates to fermentation process control, in particular a two-stage pH control strategy, which is applied to the fermentation production of arachidonic acid. Background technique [0002] Arachidonic acid (C20:4, referred to as ARA), belongs to the omega-6 series of polyunsaturated fatty acids (PUFAs), plays a very important role in maintaining the normal physiological activities of the human body. It is not only an extremely important structural lipid widely present in mammalian tissues and organs, but also an important precursor substance for human prostaglandin synthesis. It has a wide range of biological activities and important nutritional effects. It has been used in food, medicine and The cosmetics field is widely used. In addition, arachidonic acid is indispensable to the neurological and physiological development of infants, and is of great significance to the growth and development and the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/645
CPCC12P7/6427
Inventor 常明王兴国金青哲刘睿杰黄健花王小三李翔宇
Owner JIANGNAN UNIV
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