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Method for performing bio-enzyme immobilization through rice bran

A technology of biological enzymes and rice bran, applied in the direction of immobilization on/in organic carriers, chemical instruments and methods, biological water/sewage treatment, etc., to achieve the effect of wide source of raw materials, low production cost and good effect

Active Publication Date: 2015-05-27
HANGZHOU CHENGJIE ENVIRONMENTAL PROTECTION
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  • Method for performing bio-enzyme immobilization through rice bran

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preparation example

[0024] Take 1 liter of distilled water, adjust the pH to 8.3 with 0.5M NaOH, add 2 ml of 30% hydrogen peroxide, and heat the water to 35°C. Weigh 200g of biological enzymes and disperse them in the above aqueous solution, vibrate with a frequency of 50K Hz ultrasonic wave for 20 minutes, then centrifuge at 5000r / min for 15 minutes, take out 600ml of supernatant, and add 2ml of 0.2mol / L borate buffer solution with a pH of 9.0 Dissolve the precipitate and filter out the insoluble matter to obtain 500ml of alkaline protein modified enzyme solution, weigh 5kg of rice bran, spray the enzyme solution evenly on the rice bran, roll while spraying, add 55g of polymerization initiator after spraying, and continue stirring for half a day. Slowly dry at 35° C. after 3 hours, and claim its water content as 3% after 3 hours.

[0025] Implementation step 1

[0026] Take 20L water from the regulating tank of a printing and dyeing factory (water quality COD cr 1500mg / L-1800mg / L, pH8.0), addi...

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Abstract

The invention discloses a bio-enzyme immobilization method in the field of sewage treatment process, and specifically discloses a method for performing bio-enzyme immobilization through utilizing rice processing product rice bran. The method comprises the following steps: processing bio-enzyme, namely, adding hydrogen peroxide to an alkaline solution, mixing bio-enzyme with a NaOH water solution at the temperature of 30 to 35 DEG C, and then collecting supernate; adding a borate buffer solution to dissolve and precipitate; filtering to obtain alkaline protein modified enzyme liquid; mixing the alkaline protein modified enzyme liquid with rice bran in a proportion; spraying the alkaline protein modified enzyme liquid on the rice bran; and slowly drying so as to obtain the rice bran immobilization bio-enzyme. The method has the advantages of low production cost, wide raw material source and good sewage treatment effect.

Description

technical field [0001] The invention relates to a method for immobilizing biological enzymes in the field of sewage treatment technology, in particular to a method for immobilizing biological enzymes using rice bran, a product of rice processing. technical background [0002] The application of biological enzyme technology to treat wastewater is one of the hotspots in the field of wastewater treatment. This research work is of great significance to the national "energy saving and emission reduction" industrial policy. Biological enzyme is a kind of catalyst with great ability. Enzyme can act on the complex chemical chain in the pollutant and degrade it into small molecule organic matter or CO 2 、H 2 In the treatment of inorganic substances such as O and organic substances, free radicals are formed through enzymatic reactions, and free radicals undergo chemical polymerization reactions to form polymer compound precipitates, which can be removed by filtration. Compared with...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/02C02F3/34
Inventor 童少平魏状洪夏萍朱显跃曹利刚
Owner HANGZHOU CHENGJIE ENVIRONMENTAL PROTECTION
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