Preparation method of alicyclic acid bacillus resisting monoclonal antibody

Abstract

The invention relates to a preparation method of an alicyclic acid bacillus resisting monoclonal antibody. The method comprises the following steps: (1) preparing an immunized mouse; (2) culturing myeloma cells for 3-4 days to obtain cultured mouse myeloma cells; (3) inoculating alicyclic acid bacillus in the abdominal cavity of the immunized mouse for immunization and obtaining spleen cells; dispersing the spleen cells to obtain a spleen cell precipitate; (4) centrifuging the cultured mouse myeloma cells to obtain a myeloma cell precipitate; (5) respectively suspending and mixing the spleen cell precipitate and the myeloma cell precipitate in a DMEM high-sugar culture solution, and centrifuging to obtain a precipitate; centrifuging and suspending the precipitate to obtain fused cells; (7) culturing and assaying the fused cells to determine hybridoma cells; cloning and screening the hybridoma cells to obtain a hybridoma cell line; and (8) preparing a monoclonal antibody, namely performing monoclonal antibody production on the hybridoma cell line according to a general method. The preparation method disclosed by the invention is rapid and high in specificity.

Description

technical field [0001] The invention relates to a preparation method of a monoclonal antibody, in particular to a preparation method of an anti-alicyclobacillus monoclonal antibody. Background technique [0002] China is the largest producer of apples, and concentrated apple juice is also exported in large quantities. However, due to the presence of members of the Alicyclobacillus family (Alicyclobacillus) that can cause changes in the flavor of juices and even spoilage, the detection of this bacteria has become a barrier to my country's juice export trade. , Seriously affected the export earnings of fruit juice products. At present, the detection and identification of this genus are mainly conventional traditional detection methods and modern detection methods, and the combination of the two methods is usually used for detection and identification. The traditional method includes the physiological and biochemical characteristics of the bacteria to be tested, such as morphol...

AI Technical Summary

Problems solved by technology

The traditional method includes the morphological characteristics of the bacteria to be tested, the physiological and biochemical characteristics such as the growth temperature range, etc., but the shortcomings of the traditional method, such as many detection indicators, complicated procedures, and time-consuming, cause the detection results to lag behind, and cannot quickly guide production and feed back information to the production line to take corresponding measures. preventive and control measures

In addition, the various recommended methods are not nearly the same, and the results also have certain differences.