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PEG-PLGA encapsulated hemoglobin

A PEG-PLGA, hemoglobin technology, applied in the field of hemoglobin encapsulation by a new type of hemoglobin oxygen carrier - PEG-PLGA, can solve the problems of safety and in vivo effectiveness that have not been reported, achieve good oxygen supply effect, will not cause red blood cell aggregation, The effect of good blood compatibility

Active Publication Date: 2015-05-27
ACADEMY OF MILITARY MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In 1997, Chang et al used PLA, PLGA and polyisobutycyanoacrylate mixture as a packaging material to wrap hemoglobin, with particle size distribution from 70nm to 1110nm, but its safety and in vivo effectiveness have not been reported (Chang TMS, Yu WP.Biodegradable polymer membrane containing hemoglobin for oxygen carrier.US Patent 5670173,1997.)

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0066] Example 1. Preparation of PEG-PLGA-coated hemoglobin

[0067] The preparation of PEG-PLGA encapsulated hemoglobin of the present invention comprises the following steps:

[0068] 1) Dissolve PEG-PLGA in ethyl acetate to make its concentration 20mg / mL (all 10-30mg / mL), then mix 2mL PEG-PLGA solution with 0.4mL 120mg / mL (all 60-240mg / mL) ) The aqueous solution of hemoglobin is mixed at a mass ratio of 5:6 (5:4-5:12), and ultrasonicated in an ice-water bath at 80W (80-100W) with an ultrasonic cell disruptor for 3min (3-5min can be used) ) to form colostrum;

[0069] 2) Mix 10 mL of an aqueous solution of surfactant, in this case 7 mL of a 2wt% PVA aqueous solution and 3 mL of a 2wt% F68 aqueous solution (volume ratio 7:3, volume ratio 1:1-9:1 can be used, and a single PVA can also be used) Aqueous solution), add a mixture of 10 mL of PVA aqueous solution and F68 aqueous solution to the colostrum obtained in step 1), mix, use an ultrasonic cell disrupter at 250W (200-250W...

Embodiment 2

[0071] Example 2. Detection of physical and chemical properties of PEG-PLGA encapsulated hemoglobin

[0072]The physical and chemical properties of the PEG-PLGA coated hemoglobin particles prepared in Example 1 were detected as follows:

[0073] 1. Observation of particles by transmission electron microscope

[0074] The particle morphology was observed with a transmission electron microscope (model JEM-200CX, purchased from JEOL Ltd.).

[0075] The result is as figure 1 As shown, the prepared PEG-PLGA-encapsulated hemoglobin is spherical particles, and these particles are uniformly dispersed without agglomeration. It is preliminarily confirmed that the prepared PEG-PLGA-encapsulated hemoglobin is a nanoparticle colloidal dispersion system with concentrated particle size distribution. Each PEG-PLGA coated hemoglobin particle shape and figure 1 The result was no difference.

[0076] 2. Determination of the particle size distribution of nanoparticles by dynamic light scatter...

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Abstract

The invention discloses hemoglobin oxygen carrier-PEG-PLGA encapsulated hemoglobin. The PEG-PLGA encapsulated hemoglobin is obtained by encapsulating the hemoglobin with polyethylene glycol-poly(lactide-co-glycolide) (PEG-PLGA) nanoparticles. The hemoglobin is uniform in particle size distribution, good in dispersibility and high in oxygen appetency and has good oxygen supply effect and can be used for the system with general or partial anoxia or the system needing in vitro oxygen supply. The hemoglobin oxygen carrier-PEG-PLGA encapsulated hemoglobin plays an important role in the medical field when serving as a blood substitute and has a broad application prospect.

Description

technical field [0001] The invention belongs to blood substitutes in the medical field, in particular to a novel hemoglobin oxygen carrier-PEG-PLGA encapsulated hemoglobin and its application. Background technique [0002] Blood transfusion plays a pivotal role in surgical medicine and war wounded treatment, and more and more attention has been paid to the source and safety of blood supply. Because blood substitutes have the advantages of "good versatility, high safety, and long shelf life", the research and development of blood substitutes is of great significance and has attracted much attention. Among them, hemoglobin-based oxygen carriers (HBOCs) are the research hotspots in the field of blood substitutes. They are divided into chemically modified hemoglobin, conjugates of hemoglobin and various enzymes, and hemoglobin-like structures wrapped in three types. kind. Among them, the cell-like structure wrapping hemoglobin is to wrap the outside of hemoglobin with a membra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K9/51A61K47/34A61K38/42A61P7/08A61P39/00A61P9/10A61P35/00
Inventor 周虹赵莲王瑛卢明子陈赣王权尤国兴赵敬湘王波尹玉静
Owner ACADEMY OF MILITARY MEDICAL SCI
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