Sugar-free open tissue culture rapid seedling raising method of wild panax japonicas

Abstract

The invention discloses a sugar-free open tissue culture rapid seedling raising method of of wild panax japonicas. The rapid seedling raising method comprises the following steps: A, taking the stems or leaves of wild panax japonicas seedlings, and performing surface sterilization treatment; B, inoculating explants obtained through the treatment in the step A in a panax japonicas sugar-free tissue culture initial medium, and placing in a transparent plant growth box with an upper opening to carry out initial culture; C, screening rootless seedlings cultured in the step B and washing with sterile water, and cutting the cleaned rootless seedlings into single seedlings; D, inoculating the single seedlings obtained through treatment in the step C in a panax japonicas sugar-free tissue culture subculture medium, placing in the transparent plant growth box to carry out subculture; E, transplanting the plants obtained in the step D in humus soil and vermiculite mixed soil, and cultivating in a conventional way. By the application of the method, the differentiation rate of the explant is 90% or more, the growth rate can reach 4.2, the contamination rate is extremely low, qualified and vigorous tissue culture seedlings capable of being planted can be obtained, and the survival rate of transplanting is 98% or more.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, and mainly relates to a method for rapidly growing seedlings of wild bamboo ginseng in sugar-free open tissue culture. Background technique [0002] Bamboo ginseng (Panaxjaponicus C.A.Meyer) is a perennial herb of the Araliaceae genus Ginseng. It is one of the rare and endangered "seven categories" of Chinese herbal medicines in my country. And "Southern Medicine" Panax notoginseng has the effect of promoting blood circulation and removing blood stasis, and has various biological activities such as anti-inflammatory and analgesic, immune regulation, anti-myocardial ischemia, hypoglycemic, anti-aging, anti-fatigue, anti-cancer and improving learning and memory functions. [0003] In recent years, bamboo ginseng has been widely used clinically due to its various pharmacological effects, leading to an increase in its demand. For a long time, because of its high medicinal value, bamboo gi...

AI Technical Summary

Problems solved by technology

The culture container is small, and due to the presence of sugar in the medium, the leaf surface structure is poorly developed, the opening and closing function of the stomata is not strong, the leaves are small, and the chlorophyll content is low, which ultimately inhibits and reduces the photosynthetic ability of the small plants

The environment in the container, which is very different from the natural growth of the plant, also leads to a certain disorder in the physiology and shape of the plant. Those who are difficult to adapt will suffer from delayed growth or death, and some will have weak plant growth, poor leaf stretch, and poor rooting, causing domestication. stage plants with higher mortality

Traditional tissue culture uses fluorescent lamps as the light source, and plants have low utilization of the light source