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A method for removing fermentation inhibitors in lignocellulosic prehydrolyzate

A technology of fermentation inhibitors and pre-hydrolyzed liquid, which is applied in fermentation, biofuel, etc., can solve the problems of poor detoxification effect of lignocellulosic pretreatment liquid, and achieve the effect of mild conditions, good removal effect and high removal rate

Active Publication Date: 2017-11-14
INT CENT FOR BAMBOO & RATTAN
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for removing fermentation inhibitors to solve the defect that the detoxification effect of lignocellulosic pretreatment liquid is poor

Method used

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  • A method for removing fermentation inhibitors in lignocellulosic prehydrolyzate
  • A method for removing fermentation inhibitors in lignocellulosic prehydrolyzate

Examples

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Effect test

Embodiment 1

[0031] Embodiment 1 The method for removing fermentation inhibitors in bamboo prehydrolyzate

[0032] Bamboo was pretreated with chemical dilute acid, 10 mL of the obtained hydrolyzate was taken, its pH value was adjusted to 5, placed in a centrifuge tube, and centrifuged at a speed of 12000 rpm for 10 minutes, after centrifugation, 1 mL of the supernatant was taken, Add it to a 5mL elution column equipped with 1000mg graphitized carbon, install the elution column in a solid phase extraction device, use air as the eluent, 25°C, pH value 5, adjust the elution flow rate to 0.2ml / min, receive the eluate, filter it with a 0.1 micron water filter membrane, and take the filtrate for analysis.

[0033] Wherein, the sum of the concentrations of formic acid, acetic acid, levulinic acid, furfural and 5-hydroxymethylfurfural in the supernatant was 26.2 g / L.

[0034] The graphitized carbon is granular, with a particle size of 80 mesh.

Embodiment 2

[0035] Embodiment 2 The removal method of fermentation inhibitor in wood prehydrolyzate

[0036] The method of Example 1 is used to remove the wood prehydrolyzate, the difference is: the elution flow rate is 0.5ml / min; the graphitized carbon is powdery, and the particle size is 400 mesh; the pore size of the water filter membrane is 1 micron.

Embodiment 3

[0038] The method of Example 1 is used to pre-hydrolyze the corn stalks after dilute acid pretreatment. The difference is: adjust its pH value to 6, and filter it with a 0.5 micron water filter membrane.

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Abstract

The invention relates to a deprivation method of a fermentation inhibitor in a wood fiber pre-hydrolysis liquid. The deprivation method of the method comprises the following steps: adjusting pH value of a wood fiber hydrolysis liquid to 2.0-6.0, centrifugalizing and taking out supernatant; eluting through graphitized carbon by using air as an eluant, and then filtrating by a drainage membrane filter. The method is characterized by simple process, mild conditions and excellent eluting effect. According to the deprivation method of the invention, removal rate of fermentation inhibitors in the treated pre-hydrolysis liquid can be higher than 95% while carbohydrate reduction rate is controlled within 20%. The post-deprivation pre-hydrolysis liquid can also be used in alcohol fermentation to largely reduce component loss of the wood fiber materials without environment pollution and the graphitized carbon can be recycled and repeatedly used after detoxification so that the method is especially suited to industrializing promotion and application.

Description

technical field [0001] The invention belongs to the technical field of chemical utilization of lignocellulosic raw materials, and in particular relates to a method for removing fermentation inhibitors in lignocellulosic prehydrolyzed liquid. Background technique [0002] With the increasing tension of global fossil fuels and increasingly serious environmental pollution, the energy and environmental crisis has become a major obstacle to the sustainable development of human society in the 21st century. The use of renewable biomass as an alternative to fossil fuels has become increasingly urgent and important. Biomass fuels and bio-based products are one of the important research directions. One of the effective ways is to prepare sugars from cellulose and hemicellulose in biomass raw materials, and then to prepare a series of sugar platform compounds (bio-ethanol, butanol, etc.). [0003] The cellulose, hemicellulose and lignin in the lignocellulosic raw material are closely...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/10
CPCC12P7/10Y02E50/10
Inventor 李志强江泽慧费本华
Owner INT CENT FOR BAMBOO & RATTAN
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