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Preparing and applying method of cutting propagation growth promoting agent of tea tree endogenous herbaspirillum seropedicae

A technology for cutting propagation and phytospira, which is applied in the field of preparation and application of a growth promoting agent for the cutting propagation of phytospira in tea tree, can solve the problems such as that the application of raw spiraspira is not involved, and achieves the improvement of disease resistance and overcoming functions. A single, quality-improving effect

Inactive Publication Date: 2015-05-06
HUBEI UNIV
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  • Application Information

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Problems solved by technology

However, the application of endophytic Helicospirilla in tea trees did not involve

Method used

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  • Preparing and applying method of cutting propagation growth promoting agent of tea tree endogenous herbaspirillum seropedicae
  • Preparing and applying method of cutting propagation growth promoting agent of tea tree endogenous herbaspirillum seropedicae

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Effect test

Embodiment 1

[0024] Rinse the disease-free camellia tree sample with clean water, then disinfect it with 75% alcohol for 0.5 hour, and then disinfect it with 1% mercury liter for 3 minutes. Finally rinse with sterile water 3-5 times. Take 100 μl of sterile water from the last washing material, and spread it on the beef extract-peptone medium plate (3g beef extract, 10g peptone, 5g NaCl, 1.5% agar, add water to 1000ml). After 3 days of culture at 37°C and no colonies appear, cut off the edges of the sterilized tea tree leaves and tissues, cut the internal tissues into small pieces and place them on a beef extract peptone culture plate, and obtain endophytic bacteria colonies after 48 hours of culture at 37°C . Then colony purification and bacterial taxonomic identification were carried out, and finally Endospira spirulina was obtained.

Embodiment 2

[0026] Inoculate 5mL LB liquid medium (10g tryptone, 5g yeast extract, 10g NaCl, 10μg / mL spectinomycin, add water to 1000ml) with the endogenous Helicospirilla species of tea tree preserved at -80 °C, at 150ml per minute. Shake and incubate overnight at 37°C. Inject the activated bacterial solution into fresh LB liquid medium or KB liquid medium (20 g tryptone, 1.5 g K 2 HPO 4 , 15 ml glycerol, 1M MgSO 4 , 10 μg / mL spectinomycin, add water to 1000 ml) for scale-up culture, the culture conditions are shaker speed 200 rpm, temperature 28-37 °C. After bacterial culture for 8-10 hours, OD 600 When the value is 1.2-1.5, all the bacterial culture fluid is collected, and after adding 1% glycerol, it becomes the growth promoter.

Embodiment 3

[0027] Embodiment 3: 0.5:1 diluted tea tree endophytic spirulina culture solution was used to treat 100 cuttings of Jingshan No. 1 hard green stem for 3 hours, 100% survived after cutting, and the tea seedlings after 5 months had well-developed roots and new branches. Vigorous growth, dark green leaves, significantly better than the control group (see figure 1 ).

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Abstract

The invention provides a preparing and applying method of a cutting propagation growth promoting agent of tea tree endogenous herbaspirillum seropedicae. By using biological characteristics of the tea tree endogenous herbaspirillum seropedicae, a tea tree endogenous herbaspirillum seropedicae culturing solution is prepared by virtue of fermentation of the tea tree endogenous herbaspirillum seropedicae, and is diluted according to a certain ratio and then used for performing soaking treatment on a tea tree cutting wood. Plant hormones secreted by bacteria in the growth promoting agent are used for inducing the cutting wood to form adventitious roots; the bacteria in the growth promoting agent enter the cutting wood to perform endosymbiosis and continuously secret metabolites, so that quick growth of tea seedlings is promoted. The treated and cut cutting wood is developed in root system and high in planting percent, and tea seedlings are darkly green in leaves, and grow vigorously. The technical method provided by the invention is low in cost and simple to operate, is green and safe, and has multiple effects and durable action. The growth promoting agent can be used for cutting propagation of tea trees as commercial crops and can also be used for cutting propagation of other camellia plants.

Description

technical field [0001] The invention relates to a plant propagation growth-promoting agent, in particular to a preparation and application method of a tea tree endophytic spirulina cutting propagation growth-promoting agent. Background technique [0002] Tea is an important economic crop in my country. In 2012, the planting area reached 31.7 million mu, with an annual output of 1.623 million tons, accounting for 37.8% of the world, ranking first in the world in terms of planting area and output. In addition to tea as a beverage, it has become a new development direction to develop active ingredients of tea and use them in medicine, food and chemical industry. Tea tree is a cross-pollination crop, the economic traits of sexually propagated individuals are complex, the growth difference is large, and the original characteristics of the female parent cannot be maintained generally. The cutting propagation technology of tea tree with short spikes can maintain the excellent tr...

Claims

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Application Information

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IPC IPC(8): A01N63/00A01P21/00
Inventor 王行国占桂婷李亚东饶辉福丁坤明
Owner HUBEI UNIV
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