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Rapid testing paper for testing hydrogen peroxide residue in foods

A technology for hydrogen peroxide and test paper detection, which is applied in the direction of analyzing materials through chemical reactions and observing the influence of chemical indicators on materials, etc. Long response time and other issues, to achieve the effects of shortened production cycle, low production cost, simple structure and operation

Inactive Publication Date: 2015-04-29
FUDEAN OF BEIJING TECH +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, patent 200920139941.8 has announced the rapid detection test paper of hydrogen peroxide residue developed by the potassium iodide-starch method, but the test paper has a long response time, and if the test result is not observed immediately, the color of the reactant changes quickly due to the influence of air oxidation, etc. correct judgment on the result
There is also patent 200610011568.9 which discloses the rapid detection test paper for hydrogen peroxide residue developed by the principle of enzymatic color reaction, but the test paper has a long production cycle and a short validity period, which is not conducive to the circulation and application of test paper as a commodity

Method used

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  • Rapid testing paper for testing hydrogen peroxide residue in foods
  • Rapid testing paper for testing hydrogen peroxide residue in foods
  • Rapid testing paper for testing hydrogen peroxide residue in foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] First configure reaction solution A and reaction solution B, the solvent in reaction solution A is citric acid buffer solution with a pH value of 5.0, the solute is 0.4g / L horseradish peroxidase (HRP) and 5g / L polyvinylpyrrolidone The solvent in reaction solution B is absolute ethanol, and the solute is a mixture of 1.0g / L 3,3',5,5'-tetramethylbenzidine and 1mL S20 (TWEEN80). Soak 3mm chromatographic filter paper in the reaction solution A for 10s, dry it for 45min at a vacuum degree of 100Pa and a temperature of 45°C, then soak it in the reaction solution B for 10s, and dry it at a temperature of 50°C and a vacuum degree of 100Pa. Dry for 25 minutes. Adhere the treated filter paper on the substrate and cut it into 0.55cm×7cm test strips. Sealed and stored in a dry environment at 4°C, and should not be exposed to direct sunlight.

Embodiment 2

[0032] First configure reaction solution A and reaction solution B. The solvent in reaction solution A is citric acid buffer solution with a pH value of 5.0, the solute is 0.6g / L horseradish peroxidase (HRP) and 10g / L polyvinylpyrrolidone The solvent in reaction solution B is absolute ethanol, and the solute is a mixture of 2.0g / L 3,3',5,5'-tetramethylbenzidine and 3mL S20 (TWEEN80). Soak 3mm chromatographic filter paper in the reaction solution A for 20s, dry it for 50min at a vacuum degree of 110Pa and a temperature of 50°C, then soak it in the reaction solution B for 20s, and dry it at a temperature of 50°C and a vacuum degree of 110Pa. Dry for 30min. Adhere the treated filter paper on the substrate and cut it into 0.55cm×7cm test strips. Sealed and stored in a dry environment at 4°C, and should not be exposed to direct sunlight.

Embodiment 3

[0035]Take 20 samples of well-known brand milk purchased from supermarkets, take appropriate amount of samples in a clean dry container, take the area of ​​the chromogenic film immersed in the test paper as the standard, take it out immediately after immersion, and place the chromogenic film horizontally with the chromogenic film facing up. Semi-quantitative readings were then performed. Compare the color of the test paper with the standard color chart, and the value corresponding to the same color scale as the color scale of the color chart is the detection value of the sample. If the color of the test paper is between the two color scales, take the middle value of the two . The residual amount of hydrogen peroxide in the 20 milk samples was measured to be 0 mg / L, which was a qualified sample.

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Abstract

The invention relates to rapid testing paper for testing hydrogen peroxide residue in foods, belonging to the food safety testing field. The rapid testing paper is composed of a paper base and a piece of color developing paper, and has simple structure and can be operated simply. A piece of 3mm chromatographic filter paper is used as a carrier, and the testing paper can rapidly realize semi-quantitative detection on the hydrogen peroxide residue within 3s; the optimal manufacturing process is selected, and the production period of the testing paper is shortened greatly; an enzymatic protective reagent is added to improve the stability and the storage life of the testing paper; the testing conclusion can be acquired by visual observation, no precious instruments are required for displaying, and a user can master without specialized training; and the price is low, the production cost is low, the testing paper is disposable and does not need to be maintained and overhauled, the precision is high, the repeatability is good, and the rapid testing paper is very suitable for supervision departments to rapidly detect and monitor in real time in field.

Description

technical field [0001] The invention relates to a fast, sensitive and convenient test paper for detecting residual hydrogen peroxide in food, belonging to the field of food safety detection. Background technique [0002] The molecular formula of hydrogen peroxide is H2O2, the molecular weight is 34.01, and its aqueous solution is commonly known as hydrogen peroxide (Dioxowater). Now that the social economy is developing faster and faster, people are paying more and more attention to their health and food safety. Among them, the residual hazards of hydrogen peroxide have attracted people's attention. Now there have been many food poisoning incidents in China about eating food containing excessive hydrogen peroxide. Since the 1980s, many studies at home and abroad have shown that hydrogen peroxide is found to be carcinogenic, and is closely related to various diseases caused by active oxygen. The Ministry of Health of my country officially implemented GB2760-2011 "Usage Stand...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 黄昆仑许文涛程楠翟百强徐瑗聪董凯罗云波何景
Owner FUDEAN OF BEIJING TECH
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