A cell culture microfluidic chip with self-antibacterial function

Abstract

The invention discloses a cell-culture micro-fluidic chip with a self-antibacterial function. The cell-culture micro-fluidic chip comprises a flexible polymer surface-layer thin film, an upper-layer chip, a lower-layer chip, a micro valve and a guide pipe, wherein the flexible polymer surface-layer thin film is provided with a cell culture chamber, a culture-solution guide-pipe through hole, a waste-culture-solution micro-valve through hole, a buffer-solution guide-pipe through hole and an enrichment-cavity micro-valve through hole; the upper-layer chip is provided with a cell-culture-chamber bottom groove, a waste-culture-solution micro-valve bottom groove, a waste-culture-solution outlet, a buffer-solution guide-pipe bottom groove, a cell-separation flow channel, an enrichment cavity, an enrichment-cavity micro-valve bottom groove and a waste-separation-solution outlet; the upper surface of the lower-layer chip is paved with driving electrodes corresponding to the positions of the cell-culture-chamber bottom groove, the cell-separation flow channel and the enrichment cavity; the guide pipe comprises a culture-solution guide pipe and a buffer-solution guide pipe; and the micro valve comprises a waste-culture-solution micro valve and an enrichment-cavity micro valve. The cell-culture micro-fluidic chip disclosed by the invention has the advantages of minimization, high efficiency, low cost, repeatable use and self-antibacterial function and the like, and has large use prospect in the biomedicine.

Description

technical field [0001] The invention belongs to the technical field of microfluidic chips, and relates to a microfluidic chip with self-antibacterial function for cell culture. Background technique [0002] The development of microfabrication and microfluidic technology, especially the introduction of the concept of micro-total analysis system, has created a new research field of cell culture chips. Conventional cultured cells make more use of CO 2 It is carried out in an incubator and has been widely used in various fields such as virology, immunology, genetics, and oncology. The cell culture chip obtained by combining microfluidic chip and cell culture technology simulates the real environment of cell growth through structure and microfluidic control technology, and it is easy to control the microenvironment of cell growth, so as to control and manipulate single cells. At the same time, the small size of the culture chip is beneficial to directly observe the growth state...

AI Technical Summary

Problems solved by technology

In addition, microbial metabolism consumes a large amount of essential nutrients, and at the same time produces a variety of toxic metabolites, such as enzymes, antigens, and toxins, which further have a toxic effect on cells

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